2 × SYBR ntsuab qPCR MIX (Nrog ROX Siab)

Cov yam ntxwv ntawm cov khoom

Cov khoom no, 2 × SYBR ntsuab qPCR MIX, los hauv ib lub raj uas muaj tag nrho cov khoom siv uas xav tau rau PCR amplification thiab nrhiav tau, suav nrog Taq DNA poLymerase, SYBR ntsuab I dye, High ROX Reference Dye, dNTPs, Mg2+, thiab PCR buffer.
SYBR ntsuab kuv zas xim yog xim ntsuab fluorescent uas khi rau ob-stranded DNA (double-strand DNA, dsDNA) ob chav helix me groove cheeb tsam. SYBR ntsuab kuv fluoresces weakly nyob rau hauv lub xeev dawb, tab sis thaum nws khi rau ob-stranded DNA, nws cov fluorescence yog enhanced heev. Qhov no ua rau nws muaj peev xwm los ntsuas qhov ntau ntawm ob-stranded DNA tsim thaum lub sijhawm PCR amplification los ntawm kev ntes qhov fluorescence intensity.
ROX yog siv los ua cov xim kho kom raug rau kev hloov pauv ntawm fluorescence uas tsis muaj feem cuam tshuam rau PCR, yog li txo qhov sib txawv ntawm qhov chaw. Qhov sib txawv no tuaj yeem tshwm sim los ntawm ntau yam, xws li qhov yuam kev pipette lossis cov qauv evaporation. Cov cuab yeej ntsuas fluorescence sib txawv muaj cov kev xav tau sib txawv rau ROX, thiab cov khoom no yog qhov tsim nyog rau cov tshuaj ntsuas fluorescence uas xav tau kev kho ROX siab.