2 × SYBR ntsuab qPCR MIX (Nrog High ROX)

Khoom nta

Cov khoom no, 2 × SYBR ntsuab qPCR MIX, los nyob rau hauv ib lub raj uas muaj tag nrho cov khoom uas yuav tsum tau rau PCR amplification thiab nrhiav kom tau, xws li Taq DNA poLymerase, SYBR ntsuab kuv dye, High ROX Reference Dye, dNTPs, Mg2+, thiab PCR tsis.
SYBR ntsuab I dye yog xim ntsuab fluorescent dye uas khi rau ob-stranded DNA (ob chav-strand DNA, dsDNA) ob chav helix minor zawj cheeb tsam.SYBR ntsuab I fluoresces tsis muaj zog nyob rau hauv lub xeev dawb, tab sis ib zaug nws khi rau ob-stranded DNA. , nws cov fluorescence zoo heev. Qhov no ua rau nws muaj peev xwm txheeb xyuas tus nqi ntawm ob-stranded DNA tsim thaum lub sij hawm PCR amplification los ntawm kev soj ntsuam cov fluorescence siv.
ROX yog siv los ua cov xim kho kom raug rau cov fluorescence fluctuations tsis cuam tshuam nrog PCR, yog li txo qhov sib txawv ntawm qhov sib txawv. Qhov sib txawv no tuaj yeem tshwm sim los ntawm ntau yam, xws li pipette yuam kev lossis qauv evaporation. Cov khoom siv hluav taws xob sib txawv sib txawv muaj cov kev xav tau sib txawv rau ROX, thiab cov khoom no tsim nyog rau fluorescence quantification analyzers uas xav tau High ROX kho.