Xov Xwm - Ib Qho Kev Ntsuas Tshiab rau Kev Rho Tawm DNA Hauv Dej Ib puag ncig — Bigefei Sequencing Ua Kom Kev Tshawb Fawb Txog Kev Tshawb Fawb Sai Dua

Txoj Kev Siv Magnetic Bead Ua Tau Zoo Rau Kev Dais Teeb Meem Hauv Kev Rho DNA Hauv Dej Ib puag ncig

Hauv cov teb xws li kev tshawb fawb txog kab mob hauv ib puag ncig thiab kev saib xyuas dej qias neeg, kev rho tawm cov DNA genomic zoo yog qhov tseem ceeb rau cov ntawv thov downstream suav nrog PCR / qPCR thiab next-generation sequencing (NGS). Txawm li cas los xij, cov qauv dej hauv ib puag ncig yog qhov nyuaj heev, muaj ntau haiv neeg microbial, cov kab mob nyuaj rau lyse xws li Gram-positive bacteria, thiab cov teeb meem ntev uas cuam tshuam nrog cov txheej txheem rho tawm ib txwm muaj - xws li kev siv cov tshuaj lom thiab cov txheej txheem nyuaj - uas ua rau cov kws tshawb fawb muaj teeb meem tas li.

Tam sim no, Bigfish Sequencing qhia txog BFMP24R Magnetic Bead-Based Environmental Water Genomic DNA Extraction and Purification Kit, uas muab kev daws teeb meem rau cov teeb meem no los ntawm kev siv thev naus laus zis tshiab thiab kev tsim qauv yooj yim rau cov neeg siv.

Txheej Txheem Cej Luam Khoom

Cov khoom siv no yog ua raws li lub kaw lus buffer zoo tshaj plaws ua ke nrog cov hlaws dai nano magnetic ua haujlwm siab. Genomic DNA khi tshwj xeeb rau cov pab pawg ua haujlwm ntawm qhov chaw ntawm lub hlaws dai thiab raug cais tawm hauv qab lub zog magnetic sab nraud. Tom qab ntau kauj ruam ntxuav maj mam kom tshem tawm cov protein, ntsev, thiab lwm yam tsis huv, cov DNA genomic siab-purity thaum kawg tau eletted.

Tsim tshwj xeeb rau cov qauv dej ib puag ncig, cov khoom siv no tau rho tawm cov kab mob DNA uas tau sau los ntawm cov lim dej, suav nrog cov kab mob Gram-negative thiab Gram-positive (txog li 2 × 10⁹ cov kab mob hauv ib daim lim dej). Nws sib xws nrog cov tshuab rho tawm nucleic acid tag nrho rau kev ua tiav siab. Cov DNA uas tau rho tawm yog qhov zoo sib xws thiab tuaj yeem siv ncaj qha rau PCR/qPCR, NGS, thiab lwm yam kev siv downstream.

Cov yam ntxwv ntawm cov khoom

1. Muaj peev xwm rho tawm cov kab mob dav dav

Tshem tawm cov kab mob Gram-negative thiab Gram-positive tawm ntawm cov qauv dej, npog cov zej zog microbial uas feem ntau pom muaj nyob rau hauv dej qab zib thiab dej hiav txwv, thiab ua tau raws li ntau yam kev xav tau ntawm kev tshuaj ntsuam.

2. Kev Ntshiab Siab thiab Kev Ua Tau Zoo

Xa cov DNA nrog kev ntshiab siab, tsis muaj cov kuab paug uas tiv thaiv tau, thiab cov txiaj ntsig ruaj khov uas tsim nyog rau kev siv cov molecular ncaj qha mus rau hauv qab.

3. Kev Sib Txuas Lus Tsis Siv Neeg thiab Kev Ua Haujlwm Zoo

Sib xws tag nrho nrog Bigfish automated nucleic acid extraction systems, txhawb nqa kev ua tiav tib lub sijhawm ntawm 32 lossis 96 tus qauv, txo qis lub sijhawm ua tiav thiab txhim kho kev ua haujlwm hauv chav kuaj.

4. Kev Nyab Xeeb thiab Kev Siv Tau Zoo

Tsis tas yuav siv cov tshuaj lom organic reagents xws li phenol lossis chloroform, txo qhov kev pheej hmoo ntawm kev nyab xeeb hauv chav kuaj. Cov tshuaj reagents tseem ceeb tau ntim ua ntej hauv 96-qhov phaj, txo qhov yuam kev ntawm kev siv pipetting thiab ua kom yooj yim rau cov txheej txheem ua haujlwm.

Cov Cuab Yeej Sib Xws

Bigfish BFEX-16E

BFEX-32

BFEX-32E

BFEX-96E

Cov Txiaj Ntsig ntawm Kev Sim

Ib qho qauv dej ntws 600 mL raug lim los ntawm ib daim nyias nyias, thiab DNA raug rho tawm siv lub Bigfish magnetic bead-based environmental water genomic DNA extraction and purification kit ua ke nrog cov cuab yeej sib xws. Tom qab ntawd, cov DNA uas tau rho tawm tau raug soj ntsuam los ntawm agarose gel electrophoresis.

M: Cim1, 2: Cov qauv dej ntawm tus dej ntws

Cov Lus Qhia Txog Khoom

Lub sijhawm tshaj tawm: Lub Kaum Ob Hlis-18-2025