Accelerating txhim khu kev qha kab mob diagnostic

Kev kuaj mob qeeb rau cov kab mob sib kis ua rau cov neeg muaj kev pheej hmoo thoob plaws hauv peb lub ntiaj teb, tshwj xeeb tshaj yog nrog cov kab mob zoonotic kis ntawm cov tsiaj thiab tib neeg. Kwv yees li ntawm 75% ntawm 30 tus kab mob tshiab tau tshawb pom tib neeg tau sau tseg hauv 30 xyoo dhau los hauv xyoo 2008 yog tsiaj keeb kwm, raws li WHO tsab ntawv tshaj tawm tawm xyoo 2021.

"Peb pab neeg tau mob siab rau kev kov yeej cov teeb meem kev kuaj mob los ua haujlwm rau kev kuaj mob rau POCT ceev thiab kev siv tau hauv ob qho tib si IVD (hauv vitro) thiab tsis yog IVD, "hais tias Lianyi Xie, uas tau tsim Hangzhou Bigfish Bio-tech Co., Ltd, hauv 2017. "Peb qhov kev ntsuam xyuas qhov taw qhia (POCT) yog tsim los ua haujlwm nrawm hauv cov peev txheej-txheej txheem, thaum noj zaub mov. rau ntau hom kab mob spectrum. "

Bigfish's POCTs yog tsim los tiv thaiv zaub mov kev nyab xeeb, nrog rau kev noj qab haus huv ntawm cov tsiaj nyeg thiab cov tsiaj sib raug zoo, tshwj xeeb tshaj yog xav txog Tuam Tshoj txoj kev loj hlob ntawm cov tswv tsiaj.

Kev pom zoo sai POCT tsim, Xie piav qhia, yuav tsum ua kom muaj txiaj ntsig zoo ntawm kev tsim kho tshiab thiab tsom mus rau ib txwm siv thiab txhim khu kev lag luam amplification technology, raws li polymerase saw cov tshuaj tiv thaiv (PCR), txhawm rau txheeb xyuas cov feeb ntau ntawm nucleic acids los ntawm cov xov xwm nyuaj.

Xav txog qhov tshwm sim ntawm African swine fever (ASF) hauv Suav teb, lub tsev rau lub ntiaj teb cov nqaij npuas loj tshaj plaws thiab kev lag luam noj. Hauv 2019, ASF ua rau tuag ntau dua 43 lab npua, thiab ib ncig ntawm $ 111 nphom hauv kev poob. Expediating POCT designs cia siab rau kev sib koom tes nrog kev kawm thiab tsoom fwv lub koom haum, nrog rau cov lus tawm tswv yim los ntawm cov neeg siv, xws li Tuam Tshoj tus loj npua breeders.

"Qhov tseeb thiab rhiab heev ntawm qhov chaw kuaj mob, txawm tias nyob rau thaj chaw deb me me, yog qhov tseem ceeb rau peb cov khoom siv, uas yog tsim nyog thiab siv tau yooj yim rau txhua tus swineherd," Xie piav qhia.

Bigfish txoj haujlwm ntawm kev tiv thaiv kab mob thoob tebchaws thiab kev tshem tawm kuj tseem txuas ntxiv mus rau brucellosis, uas tseem yog cov kab mob zoonotic tshaj plaws thoob ntiaj teb, nrog rau cov kab mob hauv cov tsiaj txhu.

Bigfish tau pab txhawb POCT sai dua nyob ib ncig ntawm 4,000 cov chaw kho tsiaj thoob plaws Suav teb. Shuilin Zhu, tus thawj coj ntawm Zhejiang Small Animal Protection Association, hais ntxiv tias lub tuam txhab thev naus laus zis rau tsiaj

kev ua liaj ua teb thiab kev saib xyuas tsiaj tsis yog tsuas yog ua kom muaj txiaj ntsig zoo ntawm kev tiv thaiv thiab kev tswj hwm, tab sis kuj tseem txhim kho kev noj qab haus huv tsiaj.

Ua kom muaj kev tsim qauv tsis muaj nqi ntau dua rau cov neeg siv yog lwm qhov tseem ceeb hauv kev tsim thiab tsim khoom rau lawv cov kev ntsuam xyuas caj ces. Lawv qhov kev tshuaj ntsuam xyuas molecular GeNext tsis loj dua li lub raj mis dej, thiab hnyav 2 kilogrammes. Nws nta mesofluidic thiab microfluidic chips uas automate laborious kauj ruam los ntawm nucleic acid extraction, noob amplification rau real-time cov ntaub ntawv upload thiab tsom xam.

Tag nrho nyob rau hauv kom tsis txhob muaj peev xwm aerosol paug, lub GeNext 2.0 tam sim no nyob rau hauv loj ntau lawm yuav ua rau kom cov qauv throughput nce los ntawm 1 mus rau 16 ib puag ncig, tsom ib theem zuj zus ntawm 5 mus rau 25 ib tug khiav, tsis muaj sij hawm ntxiv los yog nqi.

"Peb cov qauv tsim GeNext 3.0 yuav txo lub sijhawm ntxiv, txhim kho nrog silicon-raws li chips, thiab koom nrog cov thev naus laus zis xws li nanopore sequencing rau cov ntsiab lus dav dav hauv kev kuaj mob ua ntej yug menyuam thiab kev kuaj mob ntxov ntxov," Xie hais. "Peb cov qauv POCT tuaj yeem siv ib hnub los ntawm leej twg, nyob qhov twg yam tsis tas yuav xav txog tus nqi."