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Nyob rau hauv cov teb ntawmkev kuaj mob hauv vitro (IVD), genotyping, thiab kev tshawb fawb txog molecular, cov qauv kuaj qhov ncauj—xws licov ntaub so ntswg hauv qhov ncauj, cov ntaub so ntswg caj pas, thiab cov qaub ncaug- siv dav rau kev kuaj nucleic acid vim lawv cov yam ntxwvyooj yim sau, tsis muaj kev phais, thiab cov txheej txheem kuaj tsis mobTxawm li cas los xij, cov qauv ntawm qhov ncauj feem ntau muajcov nucleic acids tsawgthiab feem ntau muaj kuab paug nrogcov protein thiab lwm yam impuritiesCov txheej txheem rho tawm ib txwm muaj feem ntau raug kev txom nyem los ntawmcov txheej txheem ua haujlwm nyuaj, kev ua haujlwm tsis zoo, thiab kev siv cov tshuaj lom, uas tuaj yeem ua rau muaj kev cuam tshuam loj heev rau qhov tseeb thiab kev ruaj khov ntawm cov ntawv thov downstream xws liPCR/qPCR thiab kev txheeb xyuas tiam tom ntej (NGS).
LubBFMP06 Cov Khoom Siv Rho Tawm DNA Genomic Uas Siv Cov Ntxaib Sib Nqus, tsim los ntawmHangzhou Bigfish FeiXu Biotechnology, muab ib qhokev daws teeb meem muaj kev nyab xeeb, ua haujlwm tau zoo, thiab txhim khu kev qharau kev rho tawm DNA qauv qhov ncauj. Nrog nws txoj kev tsim kho tshiab thiab cov qauv kev ua tau zoo, cov khoom siv no tau dhau los ua cov cuab yeej ntseeg siab rau ob qho chaw kuaj mob thiab chaw tshawb fawb.
Cov khoom siv BFMP06 yog tsim los nyob ib puag ncig alub kaw lus buffer uas tau kho kom zoo tshaj plawsua ke nrogCov hlaws dai hydroxyl magnetic tshwj xeeb rau DNA, tsim cov txheej txheem ua haujlwm ntxuav nucleic acid zoo heev. Tom qab cov qauv raug lysed hauv lysis buffer, cov khoom ntawm tes raug cuam tshuam thiab nucleic acids raug tso tawm. Cov pab pawg ua haujlwm ntawm qhov chaw ntawm cov hlaws sib nqus xaiv cov DNA dawb, tsim cov khoom ruaj khovCov hlaws dai sib nqus - DNA complexes.
Nyob rau hauv ib lub teb magnetic sab nraud, cov complexes raug kev txom nyemob kauj ruam ntxuav kom meejkom tshem tawm cov protein, ntsev, thiab lwm yam khoom tsis huv. Thaum kawg,DNA genomic uas muaj kev ntshiab siabyog eluted zoo siv siv lub elution buffer.
Kev piav qhia txog khoom
Cov khoom no siv lub kaw lus buffer tshwj xeeb uas tau tsim thiab ua kom zoo tshaj plaws ua ke nrogcov hlaws dai sib nqus uas khi DNA tshwj xeeb, ua kom sai adsorption, sib cais, thiab purification ntawm nucleic acids. Nws yog heev haum raukev rho tawm cov DNA genomic sai thiab zoo los ntawm cov swabs hauv qhov ncauj, cov swabs caj pas, thiab cov qauv qaub ncaug, thaum tshem tawm cov protein thiab ntsev seem.
Thaum siv ua ke nrogBigfish FeiXu cov cuab yeej rho tawm nucleic acid raws li cov hlaws dai sib nqus, cov khoom siv no zoo tagnrho raukev rho tawm sai saiCov DNA genomic uas tau ntxuav yog ntawmkev ntshiab siab thiab zoo heev, ua rau nws haum rau ntau yam kev siv downstream, suav nrogPCR/qPCR thiab NGS.
Cov yam ntxwv ntawm cov khoom
Zoo Siab
Ua tau zoo cais thiab ntxuav cov genomic DNA ntawmcov ntaub so ntswg hauv qhov ncauj, cov ntaub so ntswg caj pas, thiab cov qaub ncaug, xa khoomsiab yield thiab siab purity.
Ceev thiab Yooj Yim
Tsis tas yuav tsum tau ua centrifugation lossis nqus tsev lim dej ntau zaus. Siv tau nrog cov cuab yeej rho tawm tsis siv neeg, ua rau nws zoo tagnrho raukev ua cov qauv loj.
Muaj kev nyab xeeb thiab tsis muaj tshuaj lom
Tsis tas yuav siv cov tshuaj lom neeg organic xws liphenol los yog chloroform.
Cov Cuab Yeej Sib Xws
Bigfish FeiXu BFEX-16E
BFEX-32
BFEX-32E
BFEX-96
Cov Txiaj Ntsig ntawm Kev Sim
Cov qauv ntawm qhov ncauj (muab tso rau hauv400 μL kev txuag tshuaj) thiab cov qauv qaub ncaug (200 μL cov qaub ncaug + 200 μL cov tshuaj khaws cia) tau ua tiav siv covBigfish FeiXu Oral Swab Genomic DNA Purification KitDNA tau raug tshem tawm hauv70 μL elution bufferthiab tau soj ntsuam los ntawmagarose gel electrophoresis, raws li pom hauv daim duab hauv qab no.
M: DNA cim (2K Plus II)
Cov Lus Qhia Txog Khoom
Lub sijhawm tshaj tawm: Lub Ib Hlis-15-2026
Kev teeb tsa ntiag tug