DNA Methylation Testing ua ke nrog cov smartphones rau kev kuaj xyuas cov qog thiab cov leeg mob lukemia nrog qhov tseeb ntawm 90.0%!

Thaum ntxov paub ntawm cov qog nqaij hlav raws li kev ua kua mis yog qhov kev kuaj mob cancer tshiab nyob rau hauv kev kuaj mob cancer thaum ntxov lossis mob qog noj ntshav thaum ntxov. Nws tau siv dav ua ib qho tshiab biomarker rau kev paub thaum ntxov ntawm ntau cov malampancies, suav nrog cov qog nqaij hlav cancer, glioomas thiab gynecological hlav.

Qhov tshwm sim ntawm platforms los txheeb marthyleescation toj roob hauv pes (Methylscation toj roob hauv pes) biomarkers muaj lub peev xwm txhim kho kom muaj mob qog noj ntshav, muab cov neeg mob tso rau ntawm theem ntxov tshaj plaws.

Tsis ntev los no, cov kws tshawb nrhiav tau tsim kho kom yooj yim thiab ncaj qha rau cov neeg ua kom zoo nkauj (cyst) ua ke nrog kev ntsuas kub smartphone Kev kuaj mob thaum ntxov tuaj yeem ua hauv 15 feeb tom qab DNA rho tawm los ntawm cov qauv ntshav, nrog qhov tseeb ntawm 90.0%. Cov npe xov xwm yog kuaj pom sai sai ntawm kev mob qog noj ntshav ntawm tib neeg cov ntshav-capped unps thiab tshuab kawm tshuab smartphone.

Daim duab 1. Qhov yooj yim thiab nrawm nrawm nrawm thiab nrawm kuaj xyuas ntawm cyst / Aunps cov khoom siv tau ua tiav nyob rau hauv ob kauj ruam yooj yim.

Qhov no yog qhia hauv daim duab 1. Ua ntej, kev daws teeb meem tau siv los zom cov DNA tawg. Cyst / Aunps tau ntxiv rau cov tshuaj sib xyaw. Li qub thiab malignant DNA muaj cov khoom methylation sib txawv, uas ua rau DNA tawg nrog cov qauv sib tw sib txawv. Li qub DNA aggregates xoob thiab thaum kawg agrapates cyst / Aunps, yog li tias kev hloov pauv xim liab tuaj yeem pom nrog lub qhov muag liab qab. Hauv kev sib piv, qhov tshwj xeeb methylation profile ntawm DNA cancer DNA ua rau kev tsim cov pawg ntawm cov pob zeb loj ntawm DNA.

Dluab ntawm 96-cov phiaj zoo tau siv siv lub koob yees duab smartphone. Cancor DNA tau ntsuas los ntawm lub xov tooj smartphone nruab nrog siv lub tshuab kev kawm piv rau cov txheej txheem nto tshuaj.

Kev tshuaj xyuas mob cancer hauv cov ntshav tiag

Txhawm rau txuas ntxiv ntawm cov nqi hluav taws xob ntawm cov neeg tshawb xyuas lub platform, cov neeg kawm txoj moo zoo thov kev sib txawv ntawm ib txwm muaj ntshav thiab cov ntshav piv txwv tiag tiag. Methylation cov qauv ntawm CPG chaw epigenetically tswj cov noob qhia. Nyob rau hauv yuav luag txhua hom mob cancer, kev hloov pauv hauv DNA methylation thiab yog li nyob rau hauv cov kev nthuav qhia ntawm cov noob txheem uas tau txhawb Tumourigenesis tau raug saib rau lwm tus.

Raws li tus qauv rau lwm cov qog nqaij hlav uas cuam tshuam nrog cov methylation, cov kws tshawb fawb thiab cov neeg saib xyuas kev noj qab haus huv los tshawb xyuas qhov ua tau zoo ntawm cov pob txha caj dab phem. Cov qauv kev ua kom me me no tsuas yog kev saib xyuas cov leeg ntshav sai, tab sis kuj ua rau pom cov qog nqaij hlav kom zoo rau cov qog nqaij hlav uas siv cov qog ua kom yooj yim thiab ncaj qha.

DNA los ntawm cov ntshav kuaj los ntawm 31 Cov Neeg Mob Leukemia thiab 12 tus neeg noj qab nyob zoo tau raug tshuaj xyuas. Raws li qhia hauv lub thawv plaus hauv daim duab 2a, cov txheeb ze nqus ntawm cov qog ntshav cancer (ΔA650 / 525) yog qis dua li ntawm cov qauv sib piv. Qhov no feem ntau yog vim muaj kev txhim kho hydrophobicity coj mus rau ntom pob zeb ntawm cancer DNA, uas tiv thaiv kev sib sau ntawm cyst / Aunps. Raws li qhov tshwm sim, cov nanoparticles tau ua tiav tag nrho hauv cov khaubncaws sab nraud povtseg ntawm cov kab mob cancer, uas ua rau muaj kev sib txawv ntawm cov hlwv thiab cov kab mob DNA aggregates. Roc curves tau loj los ntawm kev sib txawv los ntawm kev hloov kho qhov pib los ntawm tus nqi tsawg kawg nkaus ntawm δA650 / 525 rau tus nqi siab tshaj plaws.

Daim duab 2. (a) Tus kwv tij Absorbance Qhov tseem ceeb ntawm Cyst / Aunps cov kev daws teeb meem uas qhia txog lub xub ntiag ntawm ib txwm (xiav) thiab cov kab mob (liab) DNA

(Da650 / 525) ntawm cov thawv ntawv; (B) Kev Ntsuam Xyuas ROC thiab Kev Ntsuam Xyuas ntawm Kev Kuaj Kab Mob. (c) Convusion Matrix rau kev kuaj mob ntawm cov neeg mob ib txwm thiab mob cancer. (d) Kev rhiab, feem ntau, tus nqi kwv yees zoo (PPV), tsis zoo tus nqi (NPV) thiab qhov tseeb ntawm txoj kev tsim tsim.

Raws li pom hauv daim duab 2B, thaj chaw hauv qab nkhaus (AUC = 0.9274) tau rau lub sensor tsim muaj siab thiab tshwj xeeb. Raws li tuaj yeem pom los ntawm lub thawv phiaj xwm, qhov qis tshaj plaws sawv cev cov ntaub ntawv DNA ib txwm tsis muaj kev sib cais ntawm pawg DNA Yog li ntawd, registic regression tau siv los sib txawv ntawm ib pawg ib txwm thiab pab pawg cancer. Muab ib pawg ntawm cov kev ywj pheej ywj pheej, nws kwv yees qhov tshwm sim ntawm kev tshwm sim muaj tshwm sim, xws li pab pawg cancer lossis ib txwm muaj. Tus neeg tau nqis tes ua txawv nyob ntawm 0 thiab 1. Qhov tshwm sim yog li ntawd qhov tshwm sim. Peb tau txiav txim siab qhov tshwm sim ntawm kev txheeb xyuas mob cancer (p) raws li δA650 / 525 raws li hauv qab no.

Qhov twg b = 5.3533, W1 = -6.965. Txog cov qauv kev faib tawm, qhov tshwm sim ntawm tsawg dua 0.5 qhia tias ib qho qauv ib txwm muaj, thaum siab dua ntawm 0.5 lossis siab dua qhia txog cov qauv mob cancer. Daim duab 2C piav txog qhov tsis meej pem Matrix tau tsim tawm los ntawm kev tawm-nws-ib leeg hla-kev siv tau, uas tau siv los siv tau qhov kev ruaj khov ntawm cov txheej txheem ntawm kev faib tawm. Daim duab 2D tshab taw qhia qhov kev ntsuas kuaj mob ntawm cov qauv, suav nrog kev paub txog tus nqi (PPV) thiab tsis zoo tus nqi (NPV).

Lub Mediaton-Raws Biosensors

Txhawm rau txuas ntxiv kev sim yam tsis siv tshuaj spectrophottometers, cov kws tshawb nrhiav siv dag cov kev daws teeb meem thiab paub qhov txawv ntawm cov tib neeg ib txwm thiab mob kheesxaws. Muab qhov no, lub zeem muag computer tau siv los txhais cov xim ntawm cov dna cyst / Aunps) siv cov duab ntawm 96-lub koob yees duab zoo li cas los ntawm lub koob yees duab xov tooj ntawm tes. Artificial txawj ntse tuaj yeem txo cov nqi thiab txhim kho kev siv tau rau kev txhais cov xim ntawm nanoparticle daws, thiab tsis muaj kev siv ntawm lub tshuab kho mob smartware smartphone. Thaum kawg, ob lub tshuab kawm qauv, suav nrog cov hav zoov uas tau rand) thiab txhawb nqa vector tshuab (SVM) tau txais kev cob qhia los tsim cov qauv. Ob lub RF thiab SVM cov qauv yog muab cov hnoos qeev kom zoo thiab tsis zoo nrog qhov tseeb ntawm 90.0%. Qhov no qhia tau tias kev siv cov kws txawj ntse intistial intorages hauv xov tooj ntawm tes-raws biosensing yog qhov ua tau.

Daim duab 3. (a) Chav Kawm ntawm cov tshuaj uas tau sau tseg thaum lub sijhawm npaj ntawm cov qauv rau cov duab kuaj tau. (b) Piv txwv cov duab thaum lub sijhawm kuaj tau cov duab. (C) Siv cov xim xim ntawm CYST / Aunps daws hauv txhua qhov zoo ntawm 96-hmoov phaj rho tawm ntawm cov duab (b).

Siv Cylps, cov kws tshawb nrhiav tau ua tiav cov kev pom zoo yooj yim kom pom thiab muaj peev xwm paub cov ntshav DNA thaum siv cov ntshav los ntawm kev kuaj mob leukemia. Cov tsim tawm tau pom tias DNA rho tawm los ntawm cov ntshav kom sai thiab raug nqi kho mob qog nqaij hlav (3NM) hauv 15 feeb, thiab qhia qhov tseeb ntawm 95.3%. Txhawm rau kom yooj yim ua piv txwv kev sim los ntawm kev tshem tawm cov kev xav tau ntawm cov neeg siv hluav taws xob, thiab kev sib txawv ntawm cov duab xov tooj ntawm tes, thiab qhov raug mobile siv tau ntawm 90.0%.

Siv: Doi: 10.1039 / d2ra05725E