Kev rho tawm DNA hauv av/faecal sai thiab huv si, yooj yim nrog Big Fish Sequence

Av, ua ib qho chaw muaj ntau yam kab mob sib txawv, muaj ntau yam khoom siv microbial, suav nrog ntau hom microbial xws li cov kab mob bacteria, fungi, viruses, cyanobacteria, actinomycetes, protozoa thiab nematodes. Muaj ntau yam kev ua ub no metabolic thiab cov khoom siv physiological, lawv ua lub luag haujlwm tseem ceeb hauv kev voj voog ntawm cov khoom noj hauv av thiab tseem ceeb rau kev tshem tawm cov av qias neeg. Raws li ib qho ntawm cov chaw muaj ntau yam kab mob hauv ntiaj teb, kev tshawb fawb txog molecular biology ntawm cov av muaj qhov tseem ceeb tshwj xeeb. Hauv cov txheej txheem no, kev tau txais microbial DNA los ntawm cov qauv av yog thawj kauj ruam hauv kev tshawb fawb av thiab yog kauj ruam tseem ceeb tshaj plaws rau kev vam meej ntawm kev sim downstream. Txawm li cas los xij, ntxiv rau cov khoom siv microbial nplua nuj, av feem ntau muaj ntau cov metabolites (humic acid, xanthic acid thiab lwm yam humic tshuaj), uas tuaj yeem yooj yim purified ua ke nrog nucleic acids thaum lub sijhawm nucleic acid extraction txheej txheem, cuam tshuam rau PCR thiab sequencing txheej txheem downstream.Cov Ntses LojCov Khoom Siv Ntxuav DNA Genomic Av thiab Faecal Sequencing tuaj yeem rho tawm DNA genomic ntshiab thiab muaj zog heev los ntawm cov qauv humus nplua nuj xws li cov quav av, uas yog tus pab cuam muaj zog rau kev tshawb fawb txog ntau haiv neeg ntawm cov kab mob hauv av.