Ceev thiab ntshiab, yooj yim av / faecal DNA extraction nrog Big Fish Sequence

Av, raws li ib tug ntau yam ntawm ecological ib puag ncig, yog nplua nuj nyob rau hauv microbial kev pab, nrog rau ntau yam microbial hom xws li kab mob, fungi, kab mob, cyanobacteria, actinomycetes, protozoa thiab nematodes. Muaj ntau yam kev ua haujlwm hauv metabolic thiab lub cev muaj zog, lawv ua lub luag haujlwm tseem ceeb hauv kev caij tsheb kauj vab hauv av thiab yog qhov tseem ceeb rau kev tshem tawm cov kab mob hauv av. Raws li ib qho ntawm feem ntau biologically ntau haiv neeg ib puag ncig nyob rau hauv lub ntiaj teb no, molecular biology kev tshawb fawb ntawm cov av yog ib qho tseem ceeb lom zem. Hauv cov txheej txheem no, tau txais microbial DNA los ntawm cov qauv hauv av yog thawj kauj ruam hauv kev tshawb fawb av thiab cov kauj ruam tseem ceeb tshaj plaws rau kev ua tiav ntawm kev sim hauv qab. Txawm li cas los xij, ntxiv rau cov peev txheej microbial nplua nuj, av feem ntau muaj ntau cov metabolites (humic acid, xanthic acid thiab lwm yam tshuaj humic), uas tuaj yeem yooj yim purified ua ke nrog nucleic acids thaum lub sij hawm nucleic acid extraction txheej txheem, cuam tshuam rau downstream PCR thiab sequencing txheej txheem.Ntses LojSequencing Av thiab Faecal Genomic DNA Purification Kit tuaj yeem tau txais txiaj ntsig zoo thiab sai sai tshem tawm cov ntshiab thiab muaj zog heev genomic DNA los ntawm cov qauv humus-nplua nuj xws li av faeces, uas yog lub zog pab rau cov av microbial ecosystem kev tshawb fawb ntau haiv neeg.