Yuav ua li cas xaiv qhov zoo thermal cycler rau koj cov kev xav tau kev tshawb fawb

Thermal cyclersyog cov cuab yeej indispensable thaum nws los txog rau molecular biology thiab genetic kev tshawb fawb. Kuj tseem hu ua PCR (polymerase saw cov tshuaj tiv thaiv), cov cuab yeej no yog qhov tseem ceeb rau kev nthuav dav DNA, ua rau nws yog lub hauv paus ntawm ntau yam kev siv suav nrog cloning, sequencing thiab gene qhia tsom xam. Txawm li cas los xij, muaj ntau yam kev xaiv ntawm kev ua lag luam uas xaiv qhov zoo thermal cycler rau koj cov kev xav tau kev tshawb fawb tuaj yeem yog ib txoj haujlwm txaus ntshai. Nov yog qee yam tseem ceeb uas yuav tsum xav txog thaum koj xaiv.

1. Nkag siab koj cov kev xav tau tshawb fawb

Ua ntej dhia mus rau qhov tshwj xeeb ntawm cov thermal cyclers sib txawv, nws yog ib qho tseem ceeb rau kev ntsuam xyuas koj cov kev xav tau tshwj xeeb. Xav txog hom kev sim koj yuav ua. Puas yog koj siv tus qauv PCR, quantitative PCR (qPCR), lossis daim ntawv thov kev ua haujlwm siab? Txhua daim ntawv thov no yuav xav tau cov yam ntxwv sib txawv thiab muaj peev xwm ntawm thermal cycler.

2. Temperature Range thiab Uniformity

Qhov ntsuas kub ntawm thermal cycler yog qhov tseem ceeb. Feem ntau cov txheej txheem PCR xav tau cov kauj ruam denaturation ntawm kwv yees li 94-98 ° C, ib kauj ruam annealing ntawm 50-65 ° C, thiab cov kauj ruam txuas ntxiv ntawm 72 ° C. Xyuas kom tseeb tias lub thermal cycler koj xaiv tuaj yeem tswj cov kub no thiab qhov ntsuas kub tau muab faib sib npaug thoob plaws hauv lub module. Qhov kub tsis zoo sib xws tuaj yeem cuam tshuam koj qhov kev tshawb fawb los ntawm kev ua rau cov txiaj ntsig tsis sib xws.

3. Thaiv hom thiab muaj peev xwm

Thermal cyclers tuaj nyob rau hauv ntau hom qauv, nrog rau 96-zoo daim hlau, 384-zoo daim hlau, thiab txawm 1536-zoo daim hlau. Qhov kev xaiv ntawm thaiv hom yuav tsum phim koj cov kev xav tau dhau los. Yog tias koj tab tom ua qhov kev sim siab dhau los, koj yuav xav tau cov ntawv thaiv loj dua. Hloov pauv, rau qhov kev sim me me, 96-zoo phaj yuav txaus. Tsis tas li ntawd, xav txog seb koj puas xav tau kev hloov pauv hloov tau hauv ntau hom, vim qhov no tuaj yeem ua rau muaj ntau yam ntawm koj qhov kev tshawb fawb.

4. Ceev thiab Ua Haujlwm

Nyob rau niaj hnub no qhov chaw tshawb nrhiav ceev ceev, lub sijhawm yog qhov tseem ceeb. Saib rau lub thermal cycler nrog ceev cua sov thiab txias peev xwm. Qee cov qauv siab heev tuaj yeem ua tiav lub voj voog PCR hauv qab 30 feeb, ua kom nrawm nrawm rau koj cov haujlwm. Tsis tas li ntawd, cov yam ntxwv xws li hom ceev ceev lossis cov cua kub ceev kom ua tau zoo, tso cai rau koj ua cov qauv ntau dua hauv lub sijhawm tsawg.

5. Tus neeg siv interface thiab software

Tus neeg siv-phooj ywg interface yog qhov tseem ceeb rau kev ua haujlwm tau zoo. Saib rau lub thermal cycler nrog ib qho screen kov intuitive, yooj yim txoj kev xaiv, thiab preset raws tu qauv. Cov qauv siab kuj tseem tuaj nrog software uas tso cai rau kev saib xyuas lub sijhawm thiab kev tshuaj xyuas cov ntaub ntawv, uas tshwj xeeb tshaj yog rau cov ntawv thov qPCR. Xyuas kom tseeb tias cov software tau sib xws nrog koj lub tshuab uas twb muaj lawm thiab tuaj yeem ua cov ntaub ntawv tso tawm koj xav tau.

6. Kev txiav txim siab txog nyiaj txiag

Thermal cyclers sib txawv heev ntawm tus nqi, yog li nws tseem ceeb heev kom muaj peev nyiaj ua ntej koj pib yuav ib qho. Txawm hais tias nws yuav ntxias mus nrog qhov kev xaiv pheej yig tshaj plaws, xav txog cov nqi mus sij hawm ntev ntawm kev nqis peev hauv lub tshuab zoo uas ua tau raws li koj cov kev xav tau tshawb fawb. Xav txog tsis tsuas yog tus nqi pib yuav khoom xwb, tab sis kuj yog tus nqi ntawm cov khoom siv, kev saib xyuas, thiab kev hloov kho tshiab.

7. Chaw tsim tshuaj paus kev them nyiaj yug thiab Warranty

Thaum kawg, xav txog qib kev txhawb nqa thiab kev lees paub los ntawm cov chaw tsim khoom. Ib qho kev txhim khu kev qha thermal cycler yuav tsum muaj kev lees paub zoo thiab muaj kev txhawb nqa rau cov neeg siv khoom rau kev daws teeb meem thiab kev saib xyuas. Qhov no txuag koj lub sijhawm thiab cov peev txheej hauv lub sijhawm ntev.

nyob rau hauv xaus

Xaiv txoj caithermal cyclerrau koj qhov kev xav tau kev tshawb fawb yog qhov kev txiav txim siab tseem ceeb uas tuaj yeem cuam tshuam qhov ua tiav ntawm koj qhov kev sim. Los ntawm kev ua tib zoo xav txog koj cov kev xav tau tshwj xeeb, qhov kub thiab txias, hom qauv, ceev, tus neeg siv interface, pob nyiaj siv, thiab cov chaw tsim khoom txhawb nqa, koj tuaj yeem ua qhov kev xaiv paub uas yuav txhim kho koj cov peev txheej tshawb fawb thiab tau txais txiaj ntsig zoo dua. Kev nqis peev lub sij hawm hauv cov txheej txheem xaiv no yuav kawg them rau qhov zoo thiab kev ua haujlwm ntawm koj txoj haujlwm tshawb fawb.