PCR analyzer cov teeb meem: cov lus nquag nug thiab cov kev daws teeb meem

Polymerase cov tshuaj tiv thaiv (PCR) Txawm li cas los xij, zoo li ib qho cuab yeej nyuaj, ib qho pCR analyzer tuaj yeem ntsib cov teeb meem uas cuam tshuam rau nws txoj kev ua tau zoo. Kab lus no hais txog qee cov lus nug txogPccr analyzerKev daws teeb meem thiab muab cov kev daws teeb meem rau cov teeb meem.

1. Vim li cas kuv pcc kev tawm tsam tsis muaj zog loj?

Ib qho teeb meem tshwm sim feem ntau ntawm cov neeg siv yog qhov tsis muaj peev xwm ntawm pccr kev ua kom pom tseeb rau cov hom phiaj DNA. Qhov no tuaj yeem yog los ntawm ntau yam:

Tsim kom muaj kev tsim tshwj xeeb presider: Nco ntsoov tias koj cov primers qhia tshwj xeeb rau lub hom phiaj ua ntu zus thiab muaj qhov ntsuas kub zoo nkauj qis (tm). Siv cov cuab yeej software rau kev tsim qauv pib kom tsis txhob muaj cov ntaub ntawv khi.

Daws Tramplate DNA: Txheeb xyuas tias koj siv nyiaj txaus ntawm DNA. Tsawg heev yuav ua rau tsis muaj zog lossis tsis muaj kev ua kom sov.

Inhibitors nyob rau hauv cov qauv: paug hauv cov qauv tuaj yeem ua tau rau pcr cov tshuaj tiv thaiv pc. Xav txog kev ua kom huv koj cov DNA lossis siv cov qauv sib txawv.

Kev daws: Tshawb xyuas koj tus qauv tsim pib, nce cov kev txhawj xeeb, thiab nco ntsoov tias koj cov qauv tsis muaj cov tshuaj tsis pub muaj inibitors.

2. Vim li cas kuv cov ntawv pccr tsis raug?

Yog tias koj cov khoom lag luam PC tsis yog li qhov xav tau, nws tuaj yeem qhia qhov teeb meem nrog cov tshuaj tiv thaiv cov tshuaj tiv thaiv lossis cov khoom xyaw siv.

Tsis yog qhov kev ua kom nrov: qhov no tuaj yeem tshwm sim yog tias lub cim primer rau ib qho chaw tsis muaj. Kuaj xyuas qhov tshwj xeeb ntawm cov primers siv cov cuab yeej xws li tawg.

Qhov kub tsis raug kub: Yog tias qhov kub tsis muaj kub tsawg dhau, kev khi tsis yog tshwj xeeb. Kev ua kom zoo ntawm annealing kub los ntawm gradient pcr.

Kev daws: Qhov paub tseeb Primer tshwj xeeb thiab ua kom qhov kub ua kom zoo rau qhov kub thiab txias kom txhim kho qhov tseeb ntawm pr cov khoom.

3. Kuv pcR analyzer qhia cov lus yuam kev. Kuv yuav ua li cas?

Cov lus yuam kev ntawm PCR analyzer tuaj yeem muaj kev ceeb toom, tab sis lawv tuaj yeem muab cov teeb meem tshwm sim ntau rau cov teeb meem.

Kev ntsuas hluav taws xob: Nco ntsoov tias PCR analyzer yog calibrated kom raug. Kev tu xyuas thiab kev ntsuas hluav taws xob tsis sib haum yog qhov tseem ceeb kom tau txais cov txiaj ntsig raug.

Software pab pawg: Qee zaum, cov kab siv software tuaj yeem tsim teeb meem. Rov qab kho koj lub khoos phis tawm thiab tshawb xyuas rau software hloov tshiab.

Kev daws: Xa mus rau tus neeg siv phau ntawv qhia rau cov cai yuam kev thiab ua raws cov teeb meem uas pom tau zoo. Kev tu lwm tus tuaj yeem tiv thaiv ntau yam teeb meem.

4. Vim li cas kuv cov pcr cov tshuaj tiv thaiv inconsistent?

Inconsistent PCR cov txiaj ntsig tuaj yeem ua rau muaj kev ntxhov siab rau ntau yam:

Reagent Zoo: Nco ntsoov tias txhua tus reagents, suav nrog cov enzymes, buffers, thiab dntps, yog tshiab thiab ntawm siab. Kev rub tawm lossis cov tshuaj tiv thaiv kab mob yuav ua rau muaj kev hloov pauv.

Thermal Cycler Calibration: Cov chaw ntsuas kub tsis sib haum yuav cuam tshuam rau cov txheej txheem pcc. Nquag xyuas qhov kev ntsuas hluav taws xob ntawm lub thermal cycler.

Kev daws: Siv cov tshuaj reagents zoo thiab ntsuas koj lub zog cua sov thizal niaj zaus ua kom ntseeg tau tias muaj txiaj ntsig zoo ib yam nkaus.

5. Yuav ua li cas txhawm rau txhim kho pcr cov tshuaj tiv thaiv efficievy?

Txhim kho cov efficiency ntawm pccr kev tuaj yeem ua rau cov qoob loo ntau dua thiab muaj cov txiaj ntsig zoo dua.

Txhim kho cov tshuaj tiv thaiv: Sim siv cov kev sib txawv ntawm cov primers, template DNA thiab MGCL2. Txhua daim pcr kev yuav xav tau tshwj xeeb rau kev ua tau zoo tshaj plaws.

Siv cov enzymes high-falances falinity: Yog hais tias qhov tseeb yog qhov tseem ceeb, xav txog kev ua ncaj ncees ntawm kev ua txhaum kev ua haujlwm ntawm kev ua txhaum cai ua kom txo qhov yuam kev thaum lub zog.

Kev daws: Ua ib qho kev sim txhim kho kom pom kom pom cov xwm txheej zoo tshaj plaws rau koj cov teeb tsa PCR tshwj xeeb.

Hauv cov ntsiab lus

Kev daws teeb meem aPccr analyzerYuav yog txoj haujlwm tsis tu ncua, tab sis kev nkag siab txog cov teeb meem thiab lawv cov kev daws teeb meem tuaj yeem txhim kho koj cov kev paub PCR. Los ntawm kev daws cov teeb meem no los kuj tuaj yeem txhim kho cov txiaj ntsig pcr tau thiab ua kom muaj txiaj ntsig zoo hauv Molecular cov ntawv thov Biology. Kev saib xyuas ib txwm, kev xaiv ua tib zoo rau cov reagents, thiab ua kom zoo ntawm cov tshuaj tiv thaiv cov khoom siv yog kom ua tiav tsom xam pccr.