Kev ua tau zoo ntawm plaub nucleic acid amplification ua kom pom tseeb tias yog kev txheeb xyuas SARS-CE-2 hauv Ethiopia

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Qhia txog carousel ntawm peb txoj kab ib zaug. Siv cov nyees khawm yav dhau los thiab cov chaw txuas ntxiv kom txav mus los ntawm peb txoj kev nyob rau ib lub sijhawm, lossis siv cov khawm slider thaum kawg txav los ntawm peb lub sijhawm.
Txij li thaum 2019 Coronavirus kab mob (Covid-19) tshwm sim nucleic acid kev sim ua kom zoo nkauj (da dej) tau tsim los ntawm lub ntiaj teb thiab tau ua tus qauv ua haujlwm. Txawm hais tias muaj ntau qhov kev sim tau tsim tawm thiab thov kom kuaj cov kev kuaj mob tsis pom, kev ua haujlwm ntawm cov kev ntsuam xyuas no tsis tau raug soj ntsuam nyob rau ntau qhov chaw. Therefore, this study aimed to evaluate the performance of the Abbott SARS-CoV-2, Daan Gene, BGI, and Sansure Biotech assays using the Composite Reference Standard (CRS). Txoj kev tshawb no tau ua nyob rau ntawm Ethiopian Cov Qauv Kev Noj Qab Haus Huv Hauv Tebchaws tau muab rho tawm siv cov Qiaamp Ntawm 164 cov yam ntxwv, 59.1% tau zoo thiab 40.9% yog qhov tsis zoo rau CRS. Kev muag khoom Biotech Peitivity tau qis dua piv rau CRS (P <0.05). Kev muag khoom Biotech Peitivity tau qis dua piv rau CRS (P <0.05). Оложииииииииииажииииииажииииииажиииииианые резTы senseTeure Biotech Ноли Ноли Нра полнению с <CRS (P <CS (P <0,05). Cov Biotech qhov kev tshwm sim tau zoo tau ua ntau dua piv rau CRS (P <0.05).与 CRS 相比, suaxeure biotech 的阳性率显着较低 (p <0.05).与 CRS 相比, suaxeure biotech 的阳性率显着较低 (p <0.05). У Nrhiav tau Biotech Dech быачито зы знительно оренартельных равнению со ора пора пора пора п (P <0,05). Kev muag khoom Biotech tau muaj txiaj ntsig zoo dua piv rau CRS (P <0.05).Qhov kev pom zoo tag nrho ntawm plaub qhov kev txheeb xyuas yog 96.3-100% piv rau CRS. Ntxiv nrog rau qhov tsawg tshaj plaws ntawm lub sanseure biotech assay, kev ua yeeb yam ntawm plaub kev lag luam tau yuav luag sib piv. Raws li xws li, cov sansure biotech [Tshawb Fawb xwb (Ru)] xav tau kev siv tau ntxiv rau nws siv hauv Ethiopia. Thaum kawg, kev tshawb fawb ntxiv yuav tsum raug txiav txim siab los xyuas cov khoom siv uas tsim nyog cov neeg saib xyuas cov lus thov.
Kev kuaj mob yog ib feem ntawm lub Koom Haum Ntiaj Teb Kev Noj Qab Haus Huv (Leej twg) cov phiaj xwm tswv yim rau cov kab mob Corkavirus 2019 (cov tub sab thiab cov lus teb (sprp). Leej twg qhia tias cov teb chaws yuav tsum tsim kev kuaj sim ua kom tau npaj tau, kev tshawb fawb kom zoo, kev teb sai rau kev muaj kev muaj mob rau pej xeem. Qhov no qhia tau tias lub luag haujlwm ntawm lub chaw kuaj yog qhov tseem ceeb rau tus kab mob thiab kev sib kis ntawm kev sib kis tus mob thiab tswj lawv kis.
Kev kuaj mob ntawm Covid-19 kom muaj cov ntaub ntawv kev kho mob tshwm sim thiab cov tsos mob, cov tsos mob ntawm cov tsos mob / phiajcim thiab rotorchic2. Txij li thaum lub Kovid-19 qhia tawm hauv Wuhan, Suav, ntau tus lag luam nucleic acid kev ntsuam xyuas amplification (da dej) tau tsim los thoob ntiaj teb. Tiag tiag-lub sij hawm cov ntawv sau nyiaj rov qab polymerase cov tshuaj tiv thaiv (rrt-pcr) tau raug siv rau kev kuaj mob ua pa ntawm tus mob ua pa loj heev Molecular Yuav Tsum Tau Los Ntawm Sars-Cov-2 yog feem ntau raws N (Nucleocapsid Gene), e (RDRAP (RDRAP (RDRAP (RDP) cov noob hauv orf1a / B (Qhib Nyeem Ntawv Ncej 1a / B) Cov. Gene) thaj av uas pom tau los ntawm tus kab mob genome. Lawv suav hais tias yog thaj chaw tseem ceeb uas pom muaj nyob hauv tus kab mob rau tus kabmob kis tau tus mob Ntawm cov noob no, cov ntawv RDRAP thiab cov qauv muaj kev tshawb pom qhov siab nrhiav pom kev zoo siab, thaum lub txiv neej muaj cov analytical ansertical confitivity5.
Cov kev ua yeeb yam ntawm prcrays yuav sib txawv raws li ntau yam xws li: extraction reagents, kev tshuaj ntxuav ua kom zoo, zoo ntawm PCR tshuab thiab lwm yam twj paj nruag. Raws li lub Plaub Hlis 2020, ntau dua 48 kev kuaj mob sib txawv los ntawm cuaj lub teb chaws tau txais kev tso cai thaum muaj xwm ceev (EUA) rau Covid-196 Kev kuaj mob. Nyob rau hauv Ethiopia, ntau dua 14 lub zog PCR tiag tiag yog siv rau cov pob tw ntawm SARS-CE-1 ntawm 26 POLIDE M2000, Roche 48000 thiab Qe-Studio7. Ib qho ntxiv, ntau cov ntawv sau cov qhab nia muaj, xws li Daan cov cuab yeej xeem, Abbott Sars-COV-CE-xeem-xeem, thiab SARS-COV-1 BGI TEST. Txawm hais tias RRT-PC yog rhiab heev, qee tus neeg mob uas muaj cov kab mob tsis txaus ntseeg tau vim muaj cov qauv tsis txaus ntseeg vim tsis muaj kev sib sau, thauj khoom, khaws cia, thiab kuaj sim thiab kuaj thiab kuaj thiab kuaj thiab kuaj thiab kuaj thiab kuaj thiab kuaj thiab kuaj xyuas. tej yam kev mob thiab kev ua ntawm cov neeg ua hauj lwm8. Tsis tas li ntawd, cov qauv lossis tswj hwm mishandling, kev voj voog pib (CT) Yog li, nws yog qhov tseeb tias PRC cov ntawv xeem tuaj yeem paub tias cov muaj cov caj ces muaj ntau yam tiag, yog li cov kev sim siab tsuas yog txheeb xyuas cov neeg mob thiab tsis yog neeg siab ntev. Yog li ntawd, nws yog ib qho tseem ceeb kom ntsuas kev kuaj mob kuaj mob siv cov qauv txheem hauv peb qhov chaw. Txawm hais tias muaj ntau tus neeg muaj kev tiv thaiv Naat yog muaj nyob ntawm Ethiopian pej xeem Funpanance Yog li ntawd, txoj kev kawm no ua kom ntsuas qhov kev sib piv tau ntawm cov khoom lag luam muaj rau kev kuaj pom ntawm SARS-CCR siv cov qauv kuaj mob.
Tag nrho ntawm 164 tus koom nrog nrog cov uas xav tias yog cov neeg uas xav nyob hauv txoj kev kawm no. Feem ntau ntawm cov qauv tau los ntawm Chaw Kho Mob (118/164 = 72%), thaum seem 46 (28%) cov neeg tuaj koom yog los ntawm cov chaw zov me nyuam. Ntawm cov neeg koom tsis tau kho nyob rau ntawm qhov chaw, 15 (9.1%) Tau muaj mob hauv kev kho mob thiab 31 (18.9%) tau tiv toj ntawm cov ntaub ntawv paub tseeb. Cuaj caum-peb (56.7%) cov neeg koom yog txiv neej, thiab txhais tau tias (± sd) hnub nyoog ntawm cov neeg koom nrog 31.10 (± 11.82) xyoo.
Hauv txoj kev tshawb no, tus nqi zoo thiab tsis zoo ntawm plaub qhov kev xeem rau Covid-19 tau txiav txim siab. Yog li, tus nqi zoo ntawm ABBott Sars-COV-2 assay, Daan Gene, thiab Sars-1.5%, 57.5% feem ntau Cov. Cov qauv zoo thiab tsis zoo rau cov qauv (CRS) tau 97 (59.1%) thiab 67 (40.9%), ntsig txog (rooj 1). Hauv txoj kev tshawb fawb no, cov lus txhais ntawm CRS yog raws li "txoj cai zoo, uas yog qhov tshwm sim ntawm plaub qhov kev sim ua kom tau pom zoo tib yam muaj tseeb lossis tsis zoo.
Hauv txoj kev tshawb fawb no, peb pom qhov kev pom zoo tsis zoo (NPA) ntawm 100% (95% CI 94.6-100) rau txhua qhov kev ntsuas piv rau CRS. Lub sanseure biotechnology tsom xam pom minimal Ppa ntawm 93.8% (95% CIONGE 2019.4% (95% CI 96.6-99.9). Nyob rau hauv kev sib piv, tag nrho cov lus pom zoo ntawm SARS-COV-1 BGI assay thiab cov sanseure biotech 2019-ncov assay yog 98.8% thiab 96.3%, feem (Rooj 2).
Cohen's Kappa coefficient ntawm kev pom zoo ntawm CRS thiab Abbott Sars-CE-2 assay tau txais tag nrho (K = 1.00). Ib yam li ntawd, Cohen's Kappa qhov tseem ceeb pom los ntawm Daan Gene 2019-NCOV, SARS-SARS-CE-AL-NCI kuj yog tag nrho cov CRS (K ≥ 0.925). Nyob rau hauv no kev ntsuas kev sib piv, ntsuas chi-square (MCNEMAR TEXT) qhia tau tias cov khoom lag luam biotech tau raug txawv ntawm CRS cov txiaj ntsig (p = 0.031) (rooj 2).
Raws li qhia hauv Daim Duab.1 feem pua ​​ntawm tus nqi ct qis tshaj (<20 ct) ntawm Abbott Sars-CE Gene CT CT nqi (<20 ct) yog 50.3% thiab cov nqi ct siab (36-40 CT) yog 3.2%. 1 feem pua ​​ntawm tus nqi ct qis tshaj (<20 ct) ntawm Abbott Sars-CE Gene CT CT nqi (<20 ct) yog 50.3% thiab cov nqi ct siab (36-40 CT) yog 3.2%.Raws li qhia hauv Daim Duab.1, проценименимения ct ct (<20 ct) Азализа Abbott Sars-1) Ом,ниеаннилованниениениениениениена ORF1A / B АНTза Tansech 2019-ncov поцения TT ​​(<20 ct) оаачениент знакое in (36-40 CT) составляо 3,2%. 1, feem pua ​​ntawm cov nqi qis tshaj plaws tias qhov feem pua ​​ntawm cov nqi ct tsawg (<20 ct) suav nrog 50.3%, thiab siab tus nqi ct (36-40 ct) accounted rau 3.2%.如图 1 所示, Abbott Sars-CT-2 检测 (结合 (的最低) 为) 为) 为 (<20 ct) 为 (<20 检测的 检测的 检测的 检测的 检测的 检测的 基因 值显示低 值显示低 ct值 (<20 ct) 的百分比为 50.3%, 高 CT 值 (36-40 cT) 的百分比为 3.2%. Raws li muaj nyob hauv daim duab 1, qis tshaj plaws CT nqi feem pua ​​(<20 CT) ntawm RDRAP thiab NEF1A / bene CT qhia cov nyuj tsawg (<20 ct) 的 feem pua ​​yog 50.3%, 高 ct 值 (36-40 ct) 的 feem pua ​​yog 3.2%. Download Yog tias yog 1, гаализ Abbott SARS-CE-2 (соченое сроцЕнтющййй n n n n n n n n N) зроаченое значение нрначение нроачение нрначение нрначение нрначение нрначение TT (<20 ct) разotере 87,6%, з знчениениениениениениениениениениеначениениениенанчениерг гена Orf1a / b иследоваech 201 201 201 201 201 201 201 201 201 201 201 201 201 201кккккк 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 201 нокийй Низкий Окаий Raws li muaj nyob hauv daim duab 1, lub Abbott Sars-CE-2 assay (sib xyaw ua ke (<20 CT) ntawm ORF1A / B Gene hauv cov tub rog Biotech 2019 txoj kev kawm - kev tsom xam ntawm ncov qhia tau ib qho qes. Протнтнтнтй (<20 ct) составил 50,3%, а вроцений в00 составил 3,2%. Qhov feem pua ​​ntawm qhov tseem ceeb (<20 ct) yog 50.3%, thiab feem pua ​​ntawm cov nuj nqis siab (36-40-40.Lub ABBott Sars-COV-2 B xeem cov ntsiab lus CT tseem ceeb dua 30. Ntawm qhov tod tes, ntawm BGI Sars-COV-1 assay OCF1A / b noob caj ces muaj tus nqi CT (> 36 CT) feem pua ​​yog 4%. Ntawm qhov tod tes, ntawm BGI Sars-COV-1 assay OCF1A / b noob caj ces muaj tus nqi CT (> 36 CT) feem pua ​​yog 4%. С Ntawm qhov tod tes, hauv kev tsom xam ntawm BGI Sars-CEF1A / B muaj tus nqi ct siab (> 36 CT), qhov feem pua ​​ntawm uas yog 4%.另一方面, 在 BGI SARS-CARS-1 检测中, ORF1A / B 基因具有高 CT 值 (> 36 CT) 的百分比为 4% (图 1). Ntawm qhov tod tes, hauv BGI SARS-COV-COV-COV-CE-2 qhov feem pua ​​ntawm ORF1A / B Gene CT tus nqi (> 36 CT) yog 4% (Daim duab 1). С Ntawm qhov tod tes, hauv BGI SARS-COV-2 tsom xam, qhov feem pua ​​ntawm Orf1a / B noob muaj nuj nqis (> 36 CT) yog 4% (FIG.
Hauv txoj kev tshawb no, peb tau coj 164 nasopharyngeal. Rau txhua hom kev ua yeeb yam, RNA Kev Rho Tawm thiab cov Ampification tau ua rau siv cov hau kev thiab cov khoom siv pom zoo los ntawm cov tuam ntxhab.
Qhov kev kawm no qhia tau tias ABBott cov kev xeem rau SARS-CE-2 muaj tib qho kev kawm tau ua tiav raws li CRS, nrog 100% zoo, thiab tag nrho cov concordorance. Cohen's Kappa Daim Ntawv Cog Lus yog 1.00, qhia txog kev pom zoo tag nrho nrog CRS. Ib txoj kev kawm zoo sib xws los ntawm Washington hauv Asmeskas pom tias tag nrho cov kev sim ntawm ABRBOTT rau SARS-1 yog 93%, piv rau chav kuaj pom kev txiav txim siab (LDA) ntawm CDC Cov. 11. Lub ABBott Sars-COV-2 Kev tshawb nrhiav tau raws li kev sib xyaw ua ke kom pom ntawm N thiab Rdrp cov noob, raws li ob cov noob muaj ntau dua, txo qhov tsis tseeb ntawm lub ntsej muag. Kev tshawb fawb hauv Vienna, Austria kuj pom tias cov qauv kev sib tw loj thiab kev kuaj xyuas cov txiaj ntsig tsawg tsawg tsawg dua ntawm kev ua tau zoo13. Yog li, Abbott txoj kev sib tw zoo meej rau SARS-COV-COV-1 tus taw qhia tuaj yeem pom cov qauv kev sib txuas, 0.5 ml), thiab siv ntau tus nqi ntawm ELUENT (40 μl).
Peb cov txiaj ntsig tau pom tias kev kuaj pom kev ua tau zoo ntawm Daan kev kuaj cov caj ces yuav luag ib yam li CRS. Qhov no tau ua raws li kev kawm lij choj hauv Tsev Kawm Ntawv Acti hauv Huainan, Tuam Tshoj, thiab cov khw muag khoom thov 100% kev pom zoo zoo. Txawm hais tias qhia txog cov txiaj ntsig zoo ib yam, ib qho kev ua tsis zoo tom qab rov ua tib lub Elemic, tab sis yog cov biotech ncov-2019 siv. Qhov no qhia tau tias tej zaum yuav muaj kev hloov pauv hauv cov txiaj ntsig hla ntau hom kev ua yeeb yam. Txawm li cas los xij, hauv txoj kev tshawb fawb tau nqa tawm hauv Suav Teb Charonia15, tshwm sim los ntawm Daan cov noob caj ces tau sib txawv (p <0.05) piv rau lawv cov lab-txhais cov assay. Txawm li cas los xij, hauv txoj kev tshawb fawb tau nqa tawm hauv Suav Teb Charonia15, tshwm sim los ntawm Daan cov noob caj ces tau sib txawv (p <0.05) piv rau lawv cov lab-txhais cov assay. Тем Не менее, писледовании, проведении в киуоом, авзализа Taan Noob Gene Taan (P <0,05) от их их лабораторного эталоного Ализа. Txawm li cas los xij, hauv kev tshawb fawb hauv Suav teb15, Daan cov noob qhov tshwm sim tau txawv (p <0.05) los ntawm lawv qhov chaw kuaj xyuas.然而, 在中国进行的研究中 15, 大安基因检测的结果与其实验室定义的参考检测相比有显着差异 (P <0.05).然而, 在中国进行的研究中 15, 大安基因检测的结果与其实验室定义的参考检测相比有显着差 <0.05 Однако в иследовании, проведенном, ввененном, гв гитаенном, гвзуаты грезурчета Daan значительно отличались (P <0,05) О савненению с еглораторнымннммм. Txawm li cas los xij, hauv kev kawm hauv Suav Teb Charonia15, cov txiaj ntsig ntawm Daan txoj kev xeem caj ces tau sib txawv (p <0.05) piv rau nws cov kev kuaj kuaj ntsuas.Qhov tsis sib xws no tuaj yeem yog vim kev nkag siab ntawm kev ntsuas kev siv los txheeb xyuas SARS-CE-2, thiab lwm qhov kev tshawb fawb ntxiv yuav yog qhov tseem ceeb los txiav txim qhov ua rau.
Ib qho ntxiv, peb txoj kev kawm tau ntsuas qhov kev sib piv ntawm SARS-CE-2 BGI Kev Pom Zoo (NPA), thiab tag nrho cov kev pom zoo Opa). ). = 98.8%). Cohen's Kappa qhov tseem ceeb uas qhia tau cov kev pom zoo zoo (K = 0.975). Kev tshawb fawb hauv Netherlands16 thiab Tuam Tshoj [suav tau muaj cov txiaj ntsig zoo ib yam. SARS-COV-2 BGI TEST YOG IB COV GENE (ORF1A / B) Nrhiav kom tau xeem li 10 μl kev kuaj kom tau txais 10 μl. Txawm tias muaj kev pom zoo zoo kawg nkaus nrog peb cov txiaj ntsig tau, kev soj ntsuam dhau ob qho qauv zoo (1.22% ntawm tag nrho cov qauv. Qhov no tuaj yeem cuam tshuam cov chaw kuaj mob loj loj rau kev kis tau rau ntawm tus neeg mob thiab cov zej zog.
Lwm qhov kev tsom xam muaj nyob hauv qhov kev tshawb fawb no yog cov nrhiav tau Biotech NCOV-2019 rrt-pcr (ruo) assay; Tag nrho cov kev sib tw tag nrho yog 96.3%. Lub zog ntawm kev pom zoo tau txiav txim los ntawm Cohen tus nqi Kappa tus nqi, uas yog 0.925, qhia txog daim ntawv pom zoo tas nrho nrog CRS. Ib zaug ntxiv, peb cov txiaj ntsig tau zoo tib yam kev tshawb fawb tau ua nyob rau hauv Central University hauv Enginensha, Suav teb chav tsev kho mob, Lyuzhou nroog, Suav17. Txawm hais tias qhov saum toj no zoo cov ntsiab lus ntawm cov ntsiab lus txiav txim siab tau sau tseg, xeem CHI Txawm hais tias qhov saum toj no zoo cov ntsiab lus ntawm cov ntsiab lus txiav txim siab tau sau tseg, xeem CHI Несмытря Tas Nrho News зафкро 000 хазанороксировано (ккритерийй мокнемара) резора иоализа Nrhiav Tshuaj Biotech значичичичичичичичичичичич сОлнению с CS (P < 0,005). Txawm hais tias qhov kev pom zoo zoo tshaj plaws saum toj no tau sau tseg, xeem CHI尽管记录了上述良好的统计一致性, 但卡方检验 (MacNNACAR 检验) 表明, nrhiav biotech 检测的结果与 CS (P <0.005).尽管记录 了了 良好上述 良好统计 一致性统计 一致性 检验, 但 ((macnemar. ...))))))))))))))))) Несмытря Totions Los ntawm Наме ховеистичеичеиеиеиеиеиеизй хи-ки -а ((критерий пакнемара) татистически значимую рctзницу (P <0,005) Азажз Мализом Sesture BiSech thiab Crs. Txawm hais tias qhov kev cog lus zoo teev tseg saum toj no, xeem CHI-Square ntsuam xyuas (P <0.005) ntawm cov sensech assay thiab CRS.Rau rau cov qauv ntsuas (3.66%) tau pom tias tsis tseeb tsis tseeb piv rau CRS (PASTEREM 1); Qhov no tseem ceeb heev, tshwj xeeb tshaj yog muab rau kev sib kis ntawm tus kab mob ntawm tus kab mob. Cov ntaub ntawv saum toj no kuj txhawb txoj kev pom tsawg tus nqi / tus nqi.
Hauv txoj kev tshawb no, CT muaj txiaj ntsig tau txiav txim siab rau txhua qhov kev ua haujlwm ntawm lub platform thiab lub platform tsis muaj nuj nqis ct tshaj tawm hauv Abbott Sars-COV-2 assay. Qhov tshwm sim ntsig txog Abbott cov kev ua kom sib xyaw ua ke cov kev ntsuas tshuaj ntsuam xyuas cov kab mob caj ceg rau kev kuaj pom ntawm sars-cov-2. Yog li ntawd, raws li daim duab 1, 87.6% ntawm Abbott SARS-CE-2 cov txiaj ntsig tau muaj qhov ntsuas qis dua 20. CT tseem ceeb saum 30 tsis tau sau tseg. Ntxiv nrog rau Abbott siv ntawm SARS-COV-2 LUB VAJ HUAM SIB LUAG QHIA / ml. ml) 19.
Txoj kev kawm no muaj qee qhov kev txwv: ua ntej, peb tsis muaj cov qauv hloov pauv / xws li cov kab mob kis (LDA) vim tsis muaj peev txheej. Thib ob, txhua yam hnoos qeev uas siv nyob rau hauv txoj kev kawm no yog Nasopharyngeal so, thiab hom qauv, thiab thib peb, peb tus qauv loj me me.
Txoj kev kawm no piv cov kev ua tau zoo ntawm plaub RRT-PC tau ua rau SARS-CE-2 siv Nasopharyngeal kuaj. Tag nrho cov kev kuaj pom tau muaj yuav luag kev ua tau sib piv, nrog rau kev zam ntawm cov senseure biotech assay. Dhau li, qhov tsawg kawg ntawm kev kawm tau txheeb xyuas hauv cov sansure biotech assay piv rau CRS (P <0.05). Dhau li, qhov tsawg kawg ntawm kev kawm tau txheeb xyuas hauv cov sansure biotech assay piv rau CRS (P <0.05). Кроме того, a тест TraniTeure Biote Nextол полото ролоценто полоцению пОц х рОц х пОц пОц пОц (P <0,05). Tsis tas li ntawd, cov sansure biotech ntsuam xyuas tau qhia qhov feem pua ​​tsawg ntawm cov txiaj ntsig zoo piv rau CRS (P <0.05).此外, 与 CRS 相比, suaxeure biotech 检测的阳性率较低 (P <0.05).此外, 与 CRS 相比, suaxeure biotech 检测的阳性率较低 (P <0.05). Кроме того, hitter огоосureureureure Biotech болоки уоложительньх равненьх равнению с Crs (P <0,05). Tsis tas li ntawd, cov satans biotech assay muaj tus nqi qis dua piv rau CRS (P <0.05).Cov Khoom Siv Biotech NCOV-2019 (RUO) Kev tsom xam ntawm PPA, NPA thiab tag nrho kev pom zoo tshaj li 93.5% nrog lub zog Cohen Kappa lub zog ntawm kev pom zoo ntawm 0.925. Thaum kawg, cov sanseure biotech assay (RO) xav tau kev siv tau ntxiv rau hauv Ethiopia, thiab kev tshawb fawb ntxiv yuav tsum raug ntsuas los ntawm cov neeg tsim khoom.
Kev sib piv kev kawm tsim qauv nyob rau ntawm plaub lub tsev khomob hauv Addis Ababa, Eka Kote Sheetithod Center, Nillennium Lub Tsev Kho Mob Lub Tsev Kho Mob Lub Tsev Kho Mob. Cov ntaub ntawv tau sau thaum lub Kaum Ob Hlis 1 thiab 31, 2020. Cov chaw kho mob uas tau xaiv raws li lawv cov neeg muaj mob loj thiab muaj cov chaw kho mob loj hauv nroog. Zoo sib xws, seev cev, suav nrog lub ABI 7500 thiab ABBOTT M2000 REFRECT cov khoom siv, raws li feem ntau lub chaw soj ntsuam hauv Ethiopia siv tsawg kawg plaub ntawm lawv. Kuaj cov caj noob ces, ABBott Sars-COV-1 kuaj, senseure biotech xeem, thiab sars-cov-1 BGI xeem ua thaum kawm).
Kev kuaj rau SARS-COV-2 tau ua los ntawm 1 txog 30 Lub Kaum Ob Hlis 2020 Siv 3 ml ntawm Viral Thauj Khoom Nruab Nrab (Suav Nasopharyngeal kuaj tau sau los ntawm cov neeg sau cov neeg sau thiab xa mus rau Ephi hauv Triple pob. Ua ntej nucleic acid rho tawm, txhua qhov qauv yog muab ib tus lej cim npe. Extraction tau ua los ntawm txhua tus qauv tam sim thaum tuaj txog siv phau ntawv thiab tsis siv neeg cov hau kev. Yog li, rau lub tshuab ua haujlwm tsis siv neeg ntawm ABBOTT M2000, 1.3 ML (suav nrog ntawm ABBott cov qauv kev ua haujlwm (Abbott Molecular Inc. Doure, Il, USA). ) Ib pob ntawm 96 [92 cov qauv ntsuas, ob qho kev tswj hwm thiab ob qho kev tswj hwm (NTC) tau suav nrog ob ncig ntawm SARS-COUND-CUB-2 (EUA) nyob rau hauv lub sijhawm tiag tiag. Tsuas. Ib yam li ntawd, rau kev siv ntaub ntawv rho tawm, siv tib cov qauv (rau kev rho tawm tsis siv neeg thiab nrhiav pom). Yog li, thoob plaws hauv cov txheej txheem, 140 μl kuaj yog aliqueed thiab muab rho tawm siv cov qauv txhuas, lub teb chaws, ob qho tib si ntawm kev tswj hwm thiab ob lub NTCS) dua Cuaj Rounds. Manually rho tawm eluating tau nthuav dav thiab pom tau siv abi 7500 thermal cycler siv sars-1 bgi assay, thiab sense biotech assay.
Kev rho tawm cais thiab ua kom huv ntawm SARS-CE-2 kis-2 Viral RNA ua raws cov qauv sib nqus uas siv ABBott DNA npaj Reagents. Inactivation ntawm cov qauv thiab solubilization ntawm cov kab mob sib kis yog nqa tawm siv cov xab npum uas muaj guanidine ua kom tsis txhob muaj protein thiab inactivate rnase. Tus RNA yog tom qab sib cais los ntawm cov protein los ntawm cov theem sib cais siv cov sysy, piv txwv li cov lus hais txog kev ua lyhidinya txhawb txhawb nqa los ntawm nucleic acids mus rau silica (SIDO2). Cov kauj ruam yaug tshem tawm cov protein uas tseem tshuav cov protein thiab cov khib nyiab los ua cov tshuaj ntshiab. Pob tshab RNA yog cais los ntawm silica-raws li microCartict siv cov ntsuas sib nqus teb20,21. Ntawm qhov tod tes, phau ntawv kev cais thiab huv ntawm RNA yog nqa tawm los ntawm cov kab ke kem siv centrifugation thiab sib cais ntawm cov microparticts los ntawm ib qho eluent.
Lub ABBott Tiag Tiag-Time-lub sij hawm SARS-COV-2 Nrhiav kom kuaj (ABBott Molecular, uas tau txais EUA19,22 los ntawm tus uas thiab FDA. Nyob rau hauv cov qauv no, qauv qauv ua ntej kev rho tawm tau ua nyob hauv cov dej da dej ntawm 56 ° C rau 30 min. Tom qab cov kabmob kis, nucleic acid extraction tau ua rau ABBott M2000 vtm siv ABBott M2000 DNA qauv kev ua qauv. raws li cov chaw tsim khoom. Kev ua kom pom tseeb thiab nrhiav tau siv Abbott M2000 RT-PC ntsuas, thiab ob qho kev tshawb pom dual, thiab ob qho kev tshawb pom dual tau ua rau RDRAP thiab N GENES. Rox) thiab Vic p (tus xim xim) rau cov hom phiaj thiab nrhiav kom muaj kev tswj hwm sab hauv, tso cai kho kom zoo nkauj ntawm ob qho khoom lag luam hauv kev siv 19.
Lub greenification nrhiav kev pom ntawm cov khoom siv no yog raws li ib tus-kauj ruam RT-PCR Technology. ORF1A / B thiab N N Genes tau raug xaiv los ua cov cheeb tsam Daan cov noob thev naus laus zis los tshawb nrhiav thaj av ua kom pom tseeb. Cov primers tshwj xeeb thiab cov genne probes pretces nrog rau kev tshaib plab, orf1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B prob1a / B Promes sau npe los tshawb xyuas cov lus qhia-COV-2 RNA hauv cov qauv. Cov eluent kawg thiab cov tswv sib xyaw tau npaj los ntawm kev ntxiv 5 μl ntawm eluent rau 20 μl ntawm tus tswv sib xyaw rau lub sijhawm ntev ntawm 25 μl. Qhov kev ua kom nrov dua thiab nrhiav tau tau ua ib txhij ntawm lub 750024 Real-Sijhawm PCR Intrial.
Orf1a / B thiab N N Cov noob nrhiav cov khoom siv Biotech Ncov-2019 nucleic acid kuaj mob). Npaj cov kev sim tshwj xeeb rau txhua lub hom phiaj Gene los ntawm kev xaiv cov channel Fan channel rau Orf1a / B cheeb tsam thiab cov Rox channel rau cov n gene. Rau cov khoom siv ntawd, eluent thiab master mak reagents yog ntxiv raws li hauv qab no: npaj 30 μl electrication qauv thiab 20 μl edication rau kev kuaj / amplification. Tiag tiag-lub sijhawm PCR tau 750025 tau siv rau kev siv / nrhiav kom zoo.
SARS-COV-2 BGI TES yog FRORESTS REF-PCR Cov Khoom Siv RRT-PCR rau kev kuaj mob ntawm Covid-19. Lub hom phiaj cheeb tsam yog nyob rau hauv Orf1a / B cheeb tsam ntawm SARS-CEAR-1 Genome, uas yog ib txoj kev nrhiav pom. Tsis tas li ntawd, tib neeg txoj kev tu vaj tse tu vaj tse β-actin yog ib qho kev tswj hwm sab hauv sab hauv. The master mix is ​​​​prepared by mixing 20 µl of the master mix reagent and 10 µl of the extracted RNA sample in a well plate26. ABI 7500 Fluorescent Quanitive Real-Time PCR tau siv rau qhov ua kom sov thiab nrhiav kom pom. Txhua lub nucleic acid amplification, thiab kev txhais lus ntawm cov txiaj ntsig tau ua raws li cov chaw tsim khoom cov lus qhia (rooj 3).
Hauv no kev txheeb xyuas tus qauv siv los txiav txim siab feem pua ​​(qhov zoo, thiab tag nrho) thiab lwm yam kev sib piv) thiab lwm yam kev sib piv) thiab lwm yam kev sib piv rau plaub qhov kev txheeb xyuas plaub. Txhua qhov kev ntsuas sib piv tau ua tiav nrog CRS, hauv qhov kev kawm CRS tau txiav txim siab los ntawm ib qho kev xeem "thiab cov txiaj ntsig zoo, peb siv tsawg kawg ob qho kev ntsuas sib npaug. Tsis tas li ntawd, nyob rau hauv cov ntaub ntawv ntawm kev sib kis-19 kis tau, tsis tseeb tsis txaus ntshai tshaj qhov tshwm sim cuav. Yog li ntawd, hais "zoo" kom raug los ntawm CRS tshwm sim, tsawg kawg ob qhov txiaj ntsig zoo yuav los ntawm EUA Anay. Yog li, tawm ntawm plaub qhov kev sim ua, ob lossis ntau qhov kev sim ntsuas uas muab tib yam tshwm sim tib yam yog qhov muaj tseeb lossis tsis zoo [tsis zoo.
Cov ntaub ntawv tau sau siv cov qauv txheej txheem cov ntaub ntawv rho tawm, cov ntaub ntawv nkag mus thiab tsom xam tau siv Excel cov software thiab hloov kho tshiab. Qhov zoo, tsis zoo, thiab tag nrho cov lus pom zoo sib tham tau yog siv los txiav txim siab los txiav txim siab txog qib kev pom zoo ntawm txhua txoj kev muaj kev pom zoo ntawm txhua txoj kev muaj kev pom zoo ntawm txhua txoj kev nrog CRS. Kappa qhov tseem ceeb yog txhais raws li hauv qab no: 0.01 rau 0.20 rau kev pom zoo me, 0.41-0.80 rau kev pom zoo ib nrab thiab 0.81-0.99 rau kev pom zoo tiav28.
Kev coj ncaj ncees tau los ntawm University of Addis Ababa thiab txhua qhov kev sim ua rau txoj kev kawm no tau pom zoo los ntawm Ethiopian tsoomfwv kev cai saib xyuas kev cai. Tus lej siv rau Ephi Ethics Daim Ntawv Tso Cai Ethics yog Ephi / Irb-279-2020. Txhua txoj hauv kev tau thov raws li cov lus pom zoo thiab cov ntaub ntawv ntawm Ethiopian teb chaws cov lus qhia rau kev kho covb-19. Ib qho ntxiv, cov ntawv tso cai tau txais los ntawm txhua tus neeg koom nrog ua ntej koom nrog kev koom nrog hauv kev kawm.
Txhua cov ntaub ntawv tau los yog tshuaj ntsuam nyob rau hauv txoj kev kawm no muaj nyob hauv cov ntawv tshaj tawm no. Cov ntaub ntawv txhawb cov txiaj ntsig ntawm txoj kev tshawb fawb no muaj nyob ntawm tus sau sau raws li kev thov tsim nyog.
Koom Haum Saib Xyuas Kev Noj Qab Haus Huv Thoob Ntiaj Teb. Cov lus pom zoo rau kev sim kuaj cov tswv yim rau covid-19: Lub Peb Hlis 21, 2020-NCOV / Lab_20.1 (Leej twg, 2020).
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