Kev Hloov Pauv PCR: FastCycler Thermal Cycler

Hauv kev kawm txog molecular biology,cov cua sov cyclers yog ib qho cuab yeej tseem ceeb hauv cov txheej txheem polymerase chain reaction (PCR). Raws li cov kws tshawb fawb thiab cov chaw soj nstuam nrhiav kev ua haujlwm zoo thiab raug, FastCycler tau dhau los ua tus hloov pauv hauv daim teb. Nrog nws cov thev naus laus zis tshiab thiab kev ua tau zoo tshaj plaws, FastCycler tab tom teeb tsa tus qauv tshiab rau kev caij tsheb kauj vab thermal.

Lub FastCycler siv cov khoom siv Peltier zoo los ntawm Marlow, Tebchaws Meskas. Cov khoom no tau tsim los kom tswj tau qhov kub zoo heev, nrog rau qhov kub nce mus txog 6 ° C / s. Lub peev xwm nce ceev no txo ​​​​​​lub sijhawm uas xav tau rau txhua lub voj voog PCR, ua rau cov kws tshawb fawb ua tiav cov kev sim sai dua yam tsis muaj kev cuam tshuam rau qhov zoo.

Ib qho ntawm cov yam ntxwv tseem ceeb ntawm FastCycler yog nws cov voj voog suav tau zoo heev, tshaj 100 lab lub voj voog. Qhov kev ruaj khov no ua kom cov chaw kuaj mob siv tau FastCycler tau ntev, ua rau nws yog kev nqis peev uas tsim nyog rau txhua lub tsev kawm ntawv tshawb fawb. Lub neej ntev ntawm FastCycler txhais tau tias cov kws tshawb fawb tuaj yeem tsom mus rau lawv cov kev sim yam tsis tas txhawj txog kev ua tsis tiav ntawm cov khoom siv lossis qhov xav tau hloov pauv ntau zaus.

Qhov kub thiab txias raug yog qhov tseem ceeb hauv PCR, thiab FastCycler ua tau zoo heev hauv qhov no. Siv cov thev naus laus zis thermoelectric cua sov thiab txias siab heev, ua ke nrog PID (proportional-integral-derivative) kev tswj kub, FastCycler tswj tau qib kub raug thoob plaws hauv cov txheej txheem voj voog. Qhov tseeb no yog qhov tseem ceeb rau kev ua tiav cov txiaj ntsig txhim khu kev qha thiab rov ua dua, uas yog qhov tseem ceeb hauv kev tshawb fawb.

Kev sib npaug ntawm txhua lub qhov dej yog lwm qhov tseem ceeb ntawm kev hloov pauv kub, thiab FastCycler yeej tsis ua rau neeg poob siab. FastCycler tau tsim los xyuas kom meej tias qhov kub thiab txias sib xws thoob plaws txhua lub qhov dej, txo qhov kev pheej hmoo ntawm kev hloov pauv ntawm PCR cov txiaj ntsig. Qhov kev sib npaug no yog qhov tseem ceeb tshwj xeeb thaum ua haujlwm nrog cov qauv uas xav tau kev ua kom nrov meej, vim nws pab xyuas kom meej tias txhua qhov kev cuam tshuam tau ua tiav hauv tib qho xwm txheej.

Ntxiv mus, FastCycler ua haujlwm ntsiag to, ua rau nws zoo tagnrho rau cov chaw kuaj mob uas xav tau ib puag ncig ua haujlwm ntsiag to. Qhov no tso cai rau cov kws tshawb fawb ua cov kev sim yam tsis muaj kev cuam tshuam los ntawm lub suab nrov ntawm lub tshuab, tsim kom muaj huab cua zoo dua thiab muaj txiaj ntsig zoo.

Ntxiv rau nws cov lus qhia txog kev siv tshuab, FastCycler tau tsim los rau kev siv yooj yim. Nws lub interface yooj yim thiab cov kev xaiv programming yooj yim ua rau nws yooj yim rau cov kws tshawb fawb uas muaj kev paub dhau los thiab cov neeg tshiab siv. Lub peev xwm los hloov kho cov txheej txheem thiab yooj yim saib xyuas kev nce qib ua rau kev paub dhau los ntawm tus neeg siv zoo dua, tso cai rau cov kws tshawb fawb tsom mus rau lawv txoj kev tshawb fawb es tsis txhob siv lub tshuab nyuaj.

Hauv kev xaus lus, FastCyclerLub Tshuab Thermal Cyclersawv cev rau kev nce qib loj hauv PCR thev naus laus zis. Nrog nws cov khoom Peltier zoo, kev nce ceev ceev, cov ntsuas kev voj voog zoo heev, thiab kev tswj qhov kub siab heev, nws muab kev ua tau zoo tsis muaj qhov sib piv rau cov ntawv thov molecular biology. Kev sib xyaw ua ke ntawm qhov tseeb, kev sib xws, thiab kev ua haujlwm suab nrov qis ua rau FastCycler yog lub cuab yeej tseem ceeb rau txhua lub chaw kuaj mob uas tsom mus rau kev tau txais cov txiaj ntsig zoo. Raws li qhov kev thov rau PCR ceev thiab txhim khu kev qha txuas ntxiv loj hlob, FastCycler sawv tawm ua tus thawj coj hauv daim teb, ua rau cov kws tshawb fawb thawb cov ciam teb ntawm kev tshawb pom kev tshawb fawb.