Kev Hloov Pauv PCR: FastCycler Thermal Cycler

Hauv kev kawm txog molecular biology,cov cua sov cyclersyog cov cuab yeej tseem ceeb rau cov kws tshawb fawb thiab cov kws tshawb fawb. Lawv ua lub luag haujlwm tseem ceeb hauv cov txheej txheem polymerase chain reaction (PCR), uas yog lub hauv paus ntawm DNA amplification, cloning thiab ntau yam kev tshuaj xyuas genetic. Ntawm ntau lub thermal cyclers hauv khw, FastCycler sawv tawm nrog nws cov yam ntxwv siab heev thiab cov khoom zoo, ua tus qauv ntawm kev tsim kho tshiab thiab kev ua haujlwm zoo.

Lub plawv ntawm FastCycler yog nws txoj kev cog lus rau qhov zoo, siv cov khoom Peltier zoo tshaj plaws los ntawm Marlow, Tebchaws Meskas. Cov khoom no tau paub txog lawv qhov kev ntseeg siab thiab kev ua tau zoo, ua rau FastCycler ua tiav qhov kub thiab txias zoo kawg nkaus txog li 6 ° C / S. Lub peev xwm ramping sai no yog qhov tseem ceeb rau kev txo lub sijhawm tag nrho uas xav tau rau PCR cycling, ua rau cov kws tshawb fawb tau txais cov txiaj ntsig sai dua yam tsis muaj kev cuam tshuam rau kev ncaj ncees ntawm kev sim.

Ib qho ntawm cov yam ntxwv tseem ceeb ntawm FastCycler yog nws cov voj voog suav tau zoo kawg nkaus, tshaj 100 lab lub voj voog. Qhov kev ruaj khov no txhais tau tias cov kws tshawb fawb tuaj yeem siv FastCycler tau ntev, ua rau nws yog kev nqis peev uas tsim nyog rau cov chaw kuaj mob uas xav tau kev voj voog thermal tas mus li thiab rov ua dua. Lub sijhawm ntev ntawm FastCycler yog ib qho pov thawj rau nws txoj kev tsim qauv thiab kev tsim kho uas ruaj khov, ua kom ntseeg tau tias nws tuaj yeem tiv taus kev siv hauv chaw kuaj mob txhua hnub.

Qhov kub thiab txias raug yog qhov tseem ceeb heev rau kev siv PCR, thiab FastCycler ua tau zoo heev hauv qhov no. Nrog rau kev siv tshuab cua sov thiab txias thermoelectric ua ke nrog PID (proportional-integral-derivative) kev tswj kub, FastCycler tswj tau qhov kub thiab txias raug siab thoob plaws hauv kev voj voog. Qhov tseeb no yog qhov tseem ceeb rau kev ua tiav qhov zoo tshaj plaws ntawm DNA, vim tias txawm tias qhov kub hloov pauv me ntsis tuaj yeem ua rau cov txiaj ntsig tsis zoo lossis kev sim tsis ua tiav.

Kev sib npaug thoob plaws txhua lub qhov dej yog lwm qhov tseem ceeb ntawm kev hloov pauv cua sov, thiab FastCycler tsis ua rau neeg poob siab. Nws txoj kev tsim qauv ua kom ntseeg tau tias txhua tus qauv raug cua sov thiab txias tas li, uas yog qhov tseem ceeb rau kev sim uas xav tau cov xwm txheej sib npaug. Qhov kev sib npaug no txo ​​qhov sib txawv ntawm cov txiaj ntsig, ua rau cov kws tshawb fawb ntseeg siab tias lawv cov ntaub ntawv ntseeg tau thiab rov ua dua tshiab.

Ntxiv mus, FastCycler ua haujlwm ntawm qib suab nrov qis, ua rau nws zoo tagnrho rau cov chaw kuaj mob uas xav tau huab cua ntsiag to. Qhov no tsis yog tsuas yog txhim kho cov xwm txheej ua haujlwm ntawm cov kws tshawb fawb xwb, tab sis kuj ua rau kev paub hauv chaw kuaj mob muaj kev tsom mus rau thiab ua haujlwm tau zoo dua.

Hauv kev xaus lus, qhovFastCycler Thermal Cyclersawv cev rau kev nce qib loj hauv PCR thev naus laus zis. Nrog nws cov khoom Peltier zoo, cov nqi ramp ceev, cov ntsuas kev voj voog zoo heev, thiab cov txheej txheem tswj kub siab heev, nws tau tsim los ua kom tau raws li qhov xav tau ntawm kev tshawb fawb txog molecular biology niaj hnub no. Txawm hais tias koj tab tom ua qhov kev nthuav dav DNA niaj hnub lossis koom nrog kev tshawb fawb txog noob caj noob ces nyuaj, FastCycler muab kev ua tau zoo, kev ntseeg siab, thiab kev ua haujlwm zoo. Kev nqis peev hauv FastCycler txhais tau tias kev nqis peev rau yav tom ntej ntawm koj txoj kev tshawb fawb, xyuas kom koj muaj cov cuab yeej koj xav tau los thawb cov ciam teb ntawm kev tshawb pom kev tshawb fawb.