Reverutingizing pr: FastCycler Thermal Cycler

Nyob rau hauv thaj teb ntawm molecular biology,Lub caij cua sov thermalyog qhov tseem ceeb ntawm cov cuab yeej rau cov kws tshawb nrhiav thiab cov kws tshawb fawb. Lawv ua ib lub luag haujlwm tseem ceeb hauv cov tshuaj tiv thaiv polymerase (PCR) cov txheej txheem, uas yog DNA amplification, cloning thiab ntau yam caj ces. Ntawm cov ntau thermal caij nkoj ntawm kev ua lag luam, fastcycler sawv tawm nrog nws cov qib siab thiab cov khoom siv zoo, ua qauv ntawm kev tsim kho thiab kev ua tau zoo.

Ntawm lub plawv ntawm lub fastcycler yog nws txoj kev mob siab rau qhov zoo, siv cov peltier peltier cov ntsiab lus los ntawm Marlow, USA. Cov ntsiab lus no muaj koob meej rau lawv txoj kev ntseeg tau thiab kev ua tau zoo, ua rau kom ua tiav cov qib kub thiab txias txog li 6 ° C / s. Cov peev txheej nrawm no yog qhov tseem ceeb rau kev txiav txim siab pr cycling, cia cov kws tshawb nrhiav kom tau txais sai dua tsis muaj kev ncaj ncees ntawm kev sim.

Ib qho ntawm FastCycler cov khoom seem yog nws lub voj voog zoo nkauj, ntau tshaj 100 lab mus. Lub thev naus laus zis no tuaj yeem siv FastCycler tau siv sijhawm ntev, ua rau nws them nqi zog rau lub chaw ua haujlwm uas yuav tsum tau ua haujlwm txuas ntxiv thiab rov ua dua tshiab rau kev caij tsheb kauj vab. Lub FastCycler lub sijhawm ntev yog kev tsim qauv ntawm nws tus kws tsim khoom, kom muaj peev xwm ua kom nws tuaj yeem tiv thaiv lub ntsuas kev ua haujlwm txhua hnub.

Qhov raug yog qhov raug yog qhov tseem ceeb hauv pCR cov ntawv thov, thiab cov qauv nrawm rau hauv qhov no. Nrog kev txhim kho cov cua sov thermoelectric thiab txias txias nrog pid (sib koom ua ke) ntsuas kub thiab txias kom muaj cov txheej txheem qib siab. Qhov tseeb yog qhov tseem ceeb rau kev ua tiav Optimaling Amplaint ntawm DNA, raws li txawm muaj kev sib txawv me me tuaj yeem ua rau cov txiaj ntsig tsis zoo lossis sim ua tsis tiav.

Kev sib txuam thoob plaws txhua qhov dej yog lwm qhov tseem ceeb ntawm thermal cycling, thiab lub fastcycler tsis tu siab. Nws tsim kom ntseeg tau tias txhua qhov qauv yog rhuab thiab txias xwm yeem, uas yog qhov tseem ceeb rau kev sim uas yuav tsum muaj cov khaub ncaws hnav. Qhov kev sib txuam no tsis sib thooj hauv cov txiaj ntsig, muab cov kws tshawb fawb kev ntseeg siab tias lawv cov ntaub ntawv yog kev ntseeg siab thiab rov ua tau.

Tsis tas li ntawd, FastCycler ua haujlwm ntawm cov suab nrov tsawg, ua rau nws zoo tagnrho rau kev kuaj ib puag ncig uas yuav tsum muaj huab cua ntsiag to. Cov yam ntxwv no tsis tsuas yog kho cov neeg tshawb nrhiav 'kev ua haujlwm ua haujlwm, tab sis kuj ua rau kev sim ua haujlwm ntau rau kev tsom mus thiab txuag.

Hauv cov ntsiab lus, tusFastCycler Thermal Cyclersawv cev txoj kev nce qib loj hauv PCR thev naus laus zis. Nrog nws cov txiaj ntsig zoo hauv peltier, kev txhawb nqa sai sai, thiab nce qib kub ua kom tau raws li kev xav tau ntawm cov kev tshawb fawb ntawm cov niaj hnub niolic. Txawm hais tias koj tau kawm ua kom zoo tshaj plaws DNA amplated, lub fastcycler muab kev ua tau zoo, kev ntseeg tau, thiab kev ua tau zoo, thiab kev ua tau zoo. Kev nqis peev hauv lub nrawm nrawm txhais tau tias kev tshawb nrhiav yav tom ntej ntawm koj kev tshawb fawb, kom koj muaj cov cuab yeej koj yuav tsum tau thawb cov ciam ntawm kev tshawb pom ntawm kev tshawb pom ntawm kev tshawb pom ntawm kev tshawb pom ntawm kev tshawb pom ntawm kev tshawb fawb ntawm kev tshawb fawb.