Nyob rau hauv lub ntiaj teb niaj hnub hloov zuj zus ntawm kev tshawb fawb thiab kev sim, cov cuab yeej thiab cov cuab yeej siv hauv chav kuaj ua lub luag haujlwm tseem ceeb hauv kev ua tiav ntawm ntau yam haujlwm. Ib qho cuab yeej tseem ceeb xws li yog lub phaj sib sib zog nqus. Cov phaj tshwj xeeb no tau dhau los ua ib qho yuav tsum muaj nyob rau hauv ntau lub chaw soj nstuam, tshwj xeeb tshaj yog nyob rau hauv cov teb xws li molecular biology, biochemistry, thiab tshuaj nrhiav pom. Hauv qhov blog no, peb yuav tshawb txog ntau yam thiab qhov tseem ceeb ntawm cov phiaj sib sib zog nqus, lawv cov ntawv thov, thiab qhov zoo uas lawv coj los rau cov kws tshawb fawb.
Dab tsi yog qhov tob tob phaj?
A tob tob phajyog ib lub microplate nrog ntau qhov dej, txhua tus tsim los tuav cov kua dej ntau dua li tus qauv microplate. Cov phaj sib sib zog nqus feem ntau yog ua los ntawm cov yas zoo thiab tuaj nyob rau hauv ntau yam kev teeb tsa nrog cov peev txheej zoo li ntawm 1 ml txog 50 ml lossis ntau dua. Cov phiajcim no yog tsim los tso cai rau cov qauv khaws cia, sib tov, thiab tshuaj xyuas, ua rau lawv yog ib qho tseem ceeb hauv ntau qhov chaw kuaj.
Daim ntawv thov ntawm qhov tob tob phaj
Cov phaj sib sib zog nqus muaj ntau yam kev siv, suav nrog tab sis tsis txwv rau:
- Qauv Cia: Cov kws tshawb fawb feem ntau siv cov phiaj sib sib zog nqus rau lub sijhawm ntev ntawm cov qauv tshuaj lom neeg xws li DNA, RNA, cov protein, thiab cov kab lis kev cai ntawm tes. Qhov loj ntawm lub qhov dej muaj peev xwm, qhov kev nyab xeeb ntawm cov qauv tuaj yeem khaws cia yam tsis muaj kev pheej hmoo ntawm evaporation lossis kev sib kis.
- High-throughput kev tshuaj ntsuam: Hauv kev tshawb nrhiav thiab kev txhim kho tshuaj, cov phiaj sib sib zog nqus yog qhov tseem ceeb rau kev tshuaj ntsuam xyuas siab (HTS). Lawv ua rau cov kws tshawb fawb los sim ntau txhiab lub tebchaw ib txhij, ua kom nrawm rau kev txheeb xyuas cov neeg siv tshuaj muaj peev xwm.
- PCR thiab qPCR: Cov phaj sib sib zog nqus feem ntau yog siv rau cov tshuaj tiv thaiv polymerase saw (PCR) thiab ntau PCR (qPCR). Lawv tau tsim los ua kom muaj txiaj ntsig thermal cycling thiab txo qhov kev pheej hmoo ntawm kev sib kis ntawm cov qauv.
- Protein crystallization: Hauv cov txheej txheem biology, cov phiaj sib sib zog nqus yog siv rau kev sim protein crystallization. Cov qhov loj dua muab qhov chaw txaus rau kev loj hlob siv lead ua, uas yog qhov tseem ceeb rau kev tshawb fawb X-ray crystallography.
- Cell Culture: Cov phiaj sib sib zog nqus kuj tseem siv rau cov kab lis kev cai hauv ib puag ncig tswj. Lawv cov qauv tsim tso cai rau ntau lub xov tooj ntawm tes los ua kab lis kev cai ib txhij, ua kom muaj kev sib piv cov kev tshawb fawb thiab kev sim.
Qhov zoo ntawm kev siv cov phaj sib sib zog nqus
Siv cov phiaj sib sib zog nqus muaj ntau yam zoo uas ua rau kom muaj txiaj ntsig thiab raug hauv koj qhov chaw kuaj:
- Muaj peev xwm: Lub ntsiab kom zoo dua ntawm cov phaj sib sib zog nqus yog lawv lub peev xwm los tuav cov kua dej ntau dua, uas yog qhov tshwj xeeb tshaj yog rau cov kev sim uas xav tau cov qauv loj.
- Txo qhov kev pheej hmoo ntawm kev sib kis: Kev tsim cov phaj sib sib zog nqus kom txo qis qhov kev pheej hmoo ntawm kev sib kis ntawm cov qauv thiab ua kom muaj kev ntseeg siab ntawm cov txiaj ntsig kev sim.
- Compatibility nrog Automation: Ntau qhov sib sib zog nqus-zoo daim hlau yog sib xws nrog automated kua tuav systems, enabling high-throughput workflows thiab txo lub peev xwm rau tib neeg yuam kev.
- Kev siv ntau yam: Raws li tau hais dhau los, daim phiaj sib sib zog nqus tuaj yeem siv rau ntau yam kev siv, ua rau lawv muaj ntau yam cuab yeej rau cov kws tshawb fawb thoob plaws ntau yam kev qhuab qhia.
- Nqi-zoo: Los ntawm kev ua ntau yam qauv ib txhij, cov phaj sib sib zog nqus tuaj yeem txuag lub sijhawm thiab cov peev txheej, thaum kawg txuag cov nqi kev ua haujlwm.
nyob rau hauv xaus
Hauv kev xaus,daim phiaj sib sib zog nqusyog ib feem tseem ceeb ntawm kev xyaum kuaj niaj hnub no. Lawv versatility, muaj peev xwm, thiab compatibility nrog automation ua rau lawv ib tug invaluable cuab tam rau cov kws tshawb fawb nyob rau hauv ntau yam teb. Raws li kev tshawb fawb kev tshawb fawb txuas ntxiv mus, qhov tseem ceeb ntawm cov phiaj sib sib zog nqus yuav tsuas loj hlob, ua rau txoj hauv kev rau kev tshawb pom tshiab thiab kev tsim kho tshiab. Txawm hais tias koj koom nrog hauv kev tshawb nrhiav tshuaj, molecular biology, lossis lwm yam kev qhuab qhia kev tshawb fawb, kev nqis peev hauv cov phiaj xwm zoo sib sib zog nqus tuaj yeem txhim kho koj cov peev txheej tshawb fawb.
Post lub sij hawm: Dec-19-2024