Kev Siv Tau Ntau Yam ntawm Cov Phaj Qhov Dej Sib Sib Zog hauv Kev Tshawb Fawb Hauv Chav Kuaj Mob

Cov phaj qhov dej tobyog ib qho tseem ceeb hauv kev tshawb fawb hauv chav kuaj, muab cov kev daws teeb meem ntau yam thiab ua tau zoo rau ntau yam kev siv. Cov phaj ntau qhov no tau tsim los kom haum rau cov qauv hauv txoj kev ua haujlwm siab, ua rau lawv yog ib qho cuab yeej tseem ceeb hauv ntau yam kev tshawb fawb xws li genomics, proteomics, kev tshawb pom tshuaj, thiab ntau ntxiv.

Ib qho ntawm cov txiaj ntsig tseem ceeb ntawm cov phaj qhov tob yog lawv lub peev xwm los tuav cov qauv ntau ntau. Cov phaj no muaj qhov tob ntawm 2 txog 5 hli thiab tuaj yeem tuav cov qauv ntau txog li 2 ml ib qhov, ua rau lawv zoo tagnrho rau cov ntawv thov uas xav tau kev ua cov qauv ntau ntau. Qhov no yog qhov tshwj xeeb tshaj yog nyob rau hauv kev tshuaj ntsuam xyuas siab uas ntau cov qauv yuav tsum tau ua tib lub sijhawm.

Ntxiv rau qhov muaj peev xwm kuaj tau ntau, cov phaj tob tob kuj sib xws nrog ntau yam cuab yeej siv hauv chav kuaj, suav nrog cov tshuab ua kua tsis siv neeg, centrifuges, thiab cov nyeem phaj. Qhov kev sib xws no tso cai rau kev sib koom ua ke zoo rau hauv cov haujlwm hauv chav kuaj uas twb muaj lawm, ua kom cov txheej txheem yooj yim dua thiab ua kom muaj txiaj ntsig zoo dua. Txawm hais tias siv rau kev npaj cov qauv, khaws cia lossis kev tshuaj xyuas, cov phaj tob tob muab lub platform txhim khu kev qha thiab yooj yim rau kev ua cov kev sim.

Ntxiv rau, cov phaj tob-qhov muaj ntau hom ntawv, suav nrog 96-, 384-, thiab 1536-qhov teeb tsa, muab kev ywj pheej rau cov kws tshawb fawb raws li lawv cov kev xav tau tshwj xeeb. Qhov kev hloov pauv no ua rau cov phaj tob-qhov tsim nyog rau ntau yam kev siv, los ntawm kev cog qoob loo ntawm tes thiab kev loj hlob ntawm cov kab mob me me mus rau kev ua kom cov protein crystallization thiab kev tshuaj ntsuam cov tshuaj.

Qhov kev tsim cov phaj qhov tob kuj ua rau lawv zoo tagnrho rau kev khaws cia thiab khaws cia cov qauv. Lawv txoj kev tsim kho ruaj khov thiab kev sib haum xeeb nrog cov kev xaiv kaw xws li cov yeeb yaj kiab nplaum thiab cov gaskets hau ua kom cov qauv ruaj khov thiab txo qhov kev pheej hmoo ntawm kev ua qias tuaj. Qhov no ua rau cov phaj qhov tob zoo tagnrho rau kev khaws cia ntev ntawm cov qauv tshuaj lom neeg, cov tshuaj reagents thiab cov tshuaj sib xyaw, muab cov kws tshawb fawb nrog kev daws teeb meem kev tswj hwm cov qauv txhim khu kev qha.

Tsis tas li ntawd xwb, cov phaj tob tob muaj ntau yam khoom siv, suav nrog polypropylene thiab polystyrene, txhua tus muaj qhov zoo tshwj xeeb nyob ntawm daim ntawv thov. Piv txwv li, cov phaj tob polypropylene paub txog lawv cov tshuaj tiv thaiv thiab sib xws nrog ntau yam tshuaj yaj, ua rau lawv haum rau cov ntawv thov uas muaj cov tshuaj lom neeg hnyav. Cov phaj tob polystyrene, ntawm qhov tod tes, feem ntau nyiam rau lawv qhov pom tseeb, ua rau lawv zoo tagnrho rau cov ntawv thov uas xav tau kev tshuaj xyuas pom lossis kev kuaj pom fluorescence.

Hauv kev xaus,cov phaj qhov dej tobyog ib qho cuab yeej tseem ceeb hauv kev tshawb fawb hauv chav kuaj, muab kev ua haujlwm tau zoo, ua haujlwm tau zoo, thiab kev ntseeg siab rau ntau yam kev siv. Lawv lub peev xwm kuaj siab, kev sib raug zoo nrog cov cuab yeej siv hauv chav kuaj, thiab kev ywj pheej hauv cov hom ntawv thiab cov ntaub ntawv ua rau lawv muaj nqis rau cov kws tshawb fawb hauv ntau qhov chaw tshawb fawb. Txawm hais tias rau kev ua cov qauv, kev khaws cia lossis kev tshuaj xyuas, cov phaj tob tob tseem ua lub luag haujlwm tseem ceeb hauv kev txhawb nqa kev tshawb pom thiab kev tsim kho tshiab.