SARS-COV-2 Nucleic Acid kuaj tau khoom (fluorescence RT-PCR)

Khoom nta

1, High rhiab: Txwv tsis pub nrhiav (LOD)<2 × 102 Luam / ml.

2, ob chav lub hom phiaj gene: Ntes OrFrab Gene thiab n Gene ib zaug, ua raws li cov kev cai. 

3, Haum rau ntau yam Apparatus: Abi 7500 / 7500Fastfast; Roche Lightcycler480; Biorad cfx96; Peb tus kheej tus loj-bfqp96 / 48.

4, nrawm thiab yooj yim: ua ntej sib xyaw yog yooj yim siv, cov neeg muas zaub tsuas yog xav tau ntxiv enzyme thiab template. Bigfish lub nucleic acid extraction cov khoom siv tau zoo phim rau qhov kev zoo no. Los ntawm kev siv tag nrho cov tshuab rho tawm tshuab, nws tau ceev nrawm rau cov txheej txheem ntau ntawm cov qauv.

5, Bio-Kev Nyab Xeeb: Bigfish muab cov qauv tshuaj tiv thaiv cov khoom siv tau sai sai ua kom muaj kev nyab xeeb ntawm kev nyab xeeb ntawm cov ua haujlwm.