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Magapure Oryza Sativa L. Genomic DNAmer Cov Khoom Siv
Cov lus qhia luv luv
Cov khoom siv no tau txais ib qho kev tsim tshwj xeeb thiab kev ua kom zoo nkauj cov hlaws dai, uas tau tshem tawm cov khoom siv hluav taws xob tshwj xeeb xws li polyfaccharide thiab polyphenol complexes hauv cov nroj tsuag. Nws yog qhov haum rau kev rho tawm genomic DNA los ntawm cov nplooj ntoo cog. Los ntawm kev txhawb nqa kev siv Bigfish sib nqus nucleic acid extraction rau kev siv cov qauv loj me me. Qhov kev rho tawm genomic DNA muaj siab dawb huv thiab zoo zoo, thiab tuaj yeem siv dav hauv cov dej hauv nroog hauv av / QPCR, NGS thiab lwm cov kev sim tshawb fawb.
Nta ntawm cov khoom
◆ Cov tshuaj lom neeg muaj kev nyab xeeb: tsis tas yuav muaj cov organic organic xws li Phenol / chlororm
◆ Kev Siv Tsheb Loj Lawm: Nruab nrog Bigfish Acid Extraction thiab yog qhov tsim nyog rau kev rho tawm cov qauv loj loj
◆ Kev Purity High thiab Zoo Zoo: Cov khoom siv tau zoo muaj siab heev thiab tuaj yeem siv rau cov dej hauv qab, nti hybridization thiab lwm yam kev sim.
Cov Txheej Txheem Tawm Rau Extraction
Kev sampling: cov qauv tshiab txog 100 mg lossis qhov hnyav hnyav txog 30 mg
Sib tsoo: tag nraws nrog kua nitrogen lossis ib lub grinder
Kev zom zaub mov: 65 ℃ sov sov plab zom mov
Ntawm lub tshuab: Centrifuge tus supernatant thiab ntxiv nws rau lub phaj rau rho tawm
Haum tau qhov ntsuas
Bfeatlfish-32 / BFEX-32E / BFEX-96E
Kev qhia tshwj xeeb ntawm cov khoom lag luam
| Khoom npe | Miv. Tsis yog. | Yam ntim |
| Lub magaHuvOryza Sativa L.Genomic DNA Purification Cov Khoom Siv(prov ntim pob) | Bfmp23R | 32T |
| Lub magaHuvOryza Sativa L.Genomic DNA Purification Khoom Siv (Pob Tawv Pob) | Bfmp23R96 | 96T |
| Proteinase k (pzis) | Bfrs007 | 1ml /Raj (10mg / ml) |
| Rnase a(pzis) | BFRD017 | 1ml /Raj (10mg / ml) |
Kev Ceev Ntiag Tug